Saint Barnabas High School was an American all-girls, private, Roman Catholic high school located in the Woodlawn Heights section of the Bronx, New York. It also bordered the southeastern section of the city of Yonkers in neighboring Westchester County.

Founded in 1924, it operated under the authority of the Roman Catholic Archdiocese of New York until its closing in 2024.

Founded in September 1924, the school operated at its current location from 1959 until its closing. From its founding, the school was operated by the Sisters of Charity as a service to the members of the Parish of St. Barnabas. As of September 1, 2015, the school became independent of the parish.

The school chapel was renovated sometime during the tenure of Timothy S. Collins as pastor, which lasted from 1986 to 1994.

The school followed the New York State Department of Education curriculum. Senior electives included psychology and personal finance. The school was accredited by the Middle States Association of Colleges and Schools.

A majority of students went on to colleges or universities.

The class of 2015 amassed nearly $7 million in college scholarships with a 100% graduation rate. As of 2016, the senior class earned over $10 million in scholarship and grants from a vast array of colleges and universities.

Additionally, the school offered Mercy College credits for various courses.