The Vancouver Aquarium is a public aquarium located in Stanley Park in Vancouver, British Columbia, Canada. In addition to being a major tourist attraction for Vancouver, the aquarium is a centre for marine research, ocean literacy education, climate activism, conservation and marine animal rehabilitation.

The Vancouver Aquarium was one of the first facilities to incorporate professional naturalists into the galleries to interpret animal behaviours. Prior to this, at the London Zoo Fish House, naturalists James S. Bowerbank, Ray Lankester, David W. Mitchell and Philip H. Gosse (the creator of the word aquarium) had regularly held "open house" events, but the Vancouver Aquarium was the first to employ educational naturalists on a full-time basis. Aquarium research projects extend worldwide, and include marine mammal rescue and rehabilitation.

On August 9, 2010, Prime Minister Stephen Harper and B.C. Premier Gordon Campbell announced capital funding of up to $15 million. The province would donate $10 million in funding over the next three years to help pay for a planned expansion of the 54-year-old facility, Premier Gordon Campbell said. Harper added that Ottawa would hand over up to $5 million to the aquarium for infrastructure upgrades. The aquarium, however, remained nonprofit organization. The property is owned by the City of Vancouver and rented to the aquarium for $40,000 a year since 1991 (prior to which it was $1 per year).

In October 2009 the Vancouver Aquarium was designated as a Coastal America Learning Center by the US Environmental Protection Agency. As the first Learning Center in Canada, this designation is intended to strengthen the Canadian/US partnership for protecting and restoring shared ocean resources.

On August 31, 2020, the non-profit announced on Facebook that due to the financial stresses caused by the ongoing COVID-19 pandemic, it was pausing its public programming for the time being while it engages in strategic planning for the financial sustainability of its future operations. On April 15, 2021, the Aquarium announced that an agreement had been signed to transfer ownership from the Ocean Wise Conservation Association to Herschend Family Entertainment.

The Vancouver Public Aquarium Association was formed in 1950 by UBC fisheries and oceanography professors Murray Newman, Carl Lietze and Wilbert Clemens. After receiving the help of timber baron H.R. MacMillan, alderman and businessman George Cunningham and $100,000 from each of the three levels of government. (City of Vancouver, Province of British Columbia, Federal Government of Canada), it opened on June 15, 1956, with the ribbon being cut by federal Minister of Fisheries James Sinclair. Sinclair's daughter 7-year-old Margaret was also present at the ribbon cutting ceremony (she would later marry Canadian Prime Minister Pierre Elliott Trudeau and give birth to Canadian Prime Minister Justin Trudeau). In the mid-1960s, Rufus Gibbs donated $100,000 for an extension to the Vancouver Aquarium (specifically for the creation of the Rufus Gibbs Hall of sport fishes).

Officially Canada's first public aquarium, the Vancouver Aquarium has become the largest in Canada and one of the five largest in North America. The Vancouver Aquarium was the second aquarium in the world to capture and display an orca. Other whales and dolphins on display included belugas, narwhals and dolphins.

In 1975, the Vancouver Aquarium was the first aquarium accredited by the Association of Zoos and Aquariums (AZA). The aquarium is also accredited by the Canadian Association of Zoos and Aquariums (CAZA) and in 1987 was designated Canada's Pacific National Aquarium by the Canadian Federal Government.