The following is a complete list of cast members which includes both featured and repertory players. The dates given are the dates of the season in which they first appeared as a player and the season when they left. Cast members who were only featured (never promoted to contract player) are highlighted. The original cast are listed in alphabetical order and all subsequent cast members are listed in chronological order.
| Cast member | Cast appearance | Guest appearance | Season | Episodes | |||
|---|---|---|---|---|---|---|---|
| First | Last | Last | Total | Span | Total | ||
| Craig Anton | October 14, 1995 | December 13, 1997 | — | 1–3 | 3 | ||
| Bryan Callen | October 14, 1995 | May 17, 1997 | — | 1–2 | 2 | 42 | |
| David Herman | October 14, 1995 | December 13, 1997 | — | 1–3 | 3 | ||
| Orlando Jones | October 14, 1995 | May 17, 1997 | November 8, 2003 | 1 | 1–2 | 2 | |
| Phil LaMarr | October 14, 1995 | May 20, 2000 | September 20, 2003 | 1 | 1–5 | 5 | 112 |
| Artie Lange | October 14, 1995 | January 4, 1997 | May 16, 2009 | 5 | 1–2 | 2 | |
| Mary Scheer | October 14, 1995 | May 16, 1998 | — | 1–3 | 3 | ||
| Nicole Sullivan | October 14, 1995 | June 23, 2001 | May 21, 2005 | 6 | 1–6 | 6 | 141 |
| Debra Wilson | October 14, 1995 | May 17, 2003 | May 16, 2009 | 4 | 1–8 | 8 | 196 |
| Alex Borstein | September 20, 1997 | May 18, 2002 | May 16, 2009 | 4 | 3–7 | 5 | 122 |
| Tim Conlon | September 20, 1997 | — | 2–3 | 2 | |||
| Chris Hogan | September 20, 1997 | May 16, 1998 | — | 3 | 1 | ||
| Pat Kilbane | September 20, 1997 | May 20, 2000 | — | 3–5 | 3 | ||
| Lisa Kushell | September 20, 1997 | May 16, 1998 | — | 3 | 1 | ||
| Will Sasso | September 20, 1997 | May 18, 2002 | May 16, 2009 | 3 | 3–7 | 5 | 121 |
| Aries Spears | September 20, 1997 | May 21, 2005 | — | 3–10 | 8 | 198 | |
| Andrew Bowen | September 12, 1998 | May 22, 1999 | — | 4 | 1 | ||
| Mo Collins | September 12, 1998 | May 15, 2004 | May 16, 2009 | 3 | 4–9 | 6 | 150 |
| Michael McDonald | September 12, 1998 | May 17, 2008 | March 21, 2009 | 1 | 4–13 | 10 | 239 |
| Nelson Ascencio | November 27, 1999 | June 23, 2001 | — | 5–6 | 2 | ||
| Brooke Totman | January 15, 2000 | April 15, 2000 | — | 5 | 1 | ||
| Christian Duguay | October 7, 2000 | June 23, 2001 | — | 6 | 1 | ||
| Jeff Richards | October 7, 2000 | November 4, 2000 | — | 6 | 1 | ||
| Dannah Feinglass | October 14, 2000 | January 27, 2001 | — | 6 | 1 | ||
| Andrew Daly | November 4, 2000 | May 18, 2002 | — | 6–7 | 2 | ||
| Stephnie Weir | November 18, 2000 | May 6, 2006 | — | 6–11 | 6 | 120 | |
| Frank Caliendo | September 22, 2001 | May 20, 2006 | — | 7–11 | 5 | 117 | |
| Kathryn Fiore | September 29, 2001 | May 11, 2002 | — | 7 | 1 | ||
| Bobby Lee | October 6, 2001 | May 16, 2009 | — | 7–14 | 8 | 147 | |
| Taran Killam | November 10, 2001 | May 11, 2002 | — | 7 | 1 | ||
| Jill-Michele Meleán | April 27, 2002 | April 5, 2003 | — | 7–8 | 2 | ||
| Ike Barinholtz | September 14, 2002 | May 19, 2007 | — | 8–12 | 5 | 112 | |
| Josh Meyers | September 14, 2002 | May 22, 2004 | — | 8–9 | 2 | ||
| Ron Pederson | November 2, 2002 | May 21, 2005 | — | 8–10 | 3 | ||
| Simon Helberg | November 16, 2002 | February 22, 2003 | — | 8 | 1 | ||
| Paul Vogt | December 21, 2002 | May 21, 2005 | — | 8–10 | 3 | ||
| Christina Moore | January 18, 2003 | May 10, 2003 | — | 8 | 1 | ||
| Daniele Gaither | September 13, 2003 | May 20, 2006 | September 28, 1996 | 1[a] | 9–11 | 3 | |
| Gillian Vigman | September 13, 2003 | May 15, 2004 | — | 9 | 1 | ||
| Nicole Parker | November 1, 2003 | March 28, 2009 | — | 9–14 | 6 | 113 | |
| Jordan Peele | November 15, 2003 | May 17, 2008 | — | 9–13 | 5 | ||
| Melissa Paull | December 20, 2003 | February 7, 2004 | — | 9 | 1 | ||
| Keegan-Michael Key | February 7, 2004 | May 16, 2009 | — | 9–14 | 6 | 106 | |
| Crista Flanagan | February 5, 2005 | May 16, 2009 | — | 10–14 | 5 | 81 | |
| Spencer Kayden | February 26, 2005 | May 21, 2005 | — | 10 | 1 | 4 | |
| Arden Myrin | September 17, 2005 | May 16, 2009 | — | 11–14 | 4 | ||
| Frank Caeti | September 17, 2005 | May 19, 2007 | — | 11–12 | 2 | ||
| Nicole Randall Johnson | September 17, 2005 | May 19, 2007 | — | 11–12 | 2 | ||
| Lisa Donovan | February 17, 2007 | May 5, 2007 | — | 12 | 1 | ||
| Johnny A. Sanchez | November 3, 2007 | May 16, 2009 | — | 13–14 | 2 | ||
| Anjelah Johnson | November 10, 2007 | February 16, 2008 | — | 13 | 1 | ||
| Dan Oster | November 24, 2007 | May 17, 2008 | — | 13 | 1 | ||
| Daheli Hall | February 2, 2008 | May 17, 2008 | — | 13 | 1 | ||
| Erica Ash | September 13, 2008 | April 25, 2009 | — | 14 | 1 | 12 | |
| Matt Braunger | September 13, 2008 | April 25, 2009 | — | 14 | 1 | 11 | |
| Eric Price | September 13, 2008 | April 25, 2009 | — | 14 | 1 | 12 | |
| Lauren Pritchard | September 13, 2008 | April 25, 2009 | — | 14 | 1 | 11 | |
