Abilene (pronounced /ˈæbɪliːn/) is a city in and the county seat of Dickinson County, Kansas, United States. As of the 2020 census, the population of the city was 6,460. It is home of the Dwight D. Eisenhower Presidential Library and the Greyhound Hall of Fame.

In 1803, most of modern Kansas was secured by the United States as part of the Louisiana Purchase. In 1854, the Kansas Territory was organized, and in 1861 Kansas became the 34th U.S. state.

In 1857, Dickinson County was founded and Abilene began as a stage coach stop, established by Timothy Hersey and named Mud Creek. It was not until 1860 that it was named Abilene, from a passage in the Bible (Luke 3:1), meaning "grassy plains".

In 1867, the Kansas Pacific Railway (Union Pacific) pushed westward through Abilene. In the same year, Joseph G. McCoy purchased 250 acres of land north and east of Abilene, on which he built a hotel, the Drover's Cottage, stockyards equipped for 2,000 head of cattle, and a stable for their horses. The Kansas Pacific put in a spur line at Abilene that enabled the cattle cars to be loaded and sent on to their destinations. The first twenty carloads left September 5, 1867, en route to Chicago, Illinois, where McCoy was familiar with the market. The town grew quickly and became the first "cow town" of the west.

McCoy encouraged Texas cattlemen to drive their herds to his stockyards. From 1867 to 1871, the Chisholm Trail ended in Abilene, bringing in many travelers and making Abilene one of the wildest towns in the west. The stockyards shipped 35,000 head in 1867 and became the largest stockyards west of Kansas City, Kansas. In 1871, more than 5,000 cowboys herded from 600,000 to 700,000 cows to Abilene and other Kansas railheads. Another source reports 440,200 head of cattle were shipped out of Abilene from 1867 to 1871. As railroads were built further south, the end of the Chisholm Trail was slowly moved south toward Caldwell, while Kansas homesteaders concerned with cattle ruining their farm crops moved the trail west toward and past Ellsworth.

Town marshal Tom "Bear River" Smith was initially successful policing Abilene, often using only his bare hands. He survived two assassination attempts, but was murdered and decapitated on November 2, 1870. Smith wounded one of his two attackers during the shootout preceding his death, and both suspects received life in prison for the offense. He was replaced as marshal by Wild Bill Hickok in April 1871. Hickok's time in the job was short. While the marshal was standing off a crowd during a street brawl, gambler Phil Coe took two shots at Hickok, who returned fire, killing Coe, but Hickok then accidentally shot his friend and deputy Mike Williams, who was coming to his aid. Hickok lost his job two months later in December.

In 1880, Conrad Lebold built the Lebold Mansion. Lebold was one of the early town developers and bankers from 1869 through 1889. The Hersey dugout can still be seen in the cellar. The house is now a private residence. A marker outside credits the name of the town being given by opening a Bible and using the first place name pointed to.

In 1887, Atchison, Topeka and Santa Fe Railway built a branch line from Neva (3 miles (4.8 km) west of Strong City) through Abilene to Superior, Nebraska. In 1996, the Atchison, Topeka and Santa Fe Railway merged with Burlington Northern Railroad and renamed to the current BNSF Railway.