Initial Challenges and Collaboration
1929-1940
Despite recognizing the potential of penicillin, Fleming faced challenges in isolating and purifying it in sufficient quantities for clinical use. He published his findings in 1929, but it was not until the late 1930s and early 1940s that Howard Florey, Ernst Chain, and others at Oxford University successfully isolated and purified penicillin, paving the way for its mass production and clinical application.