Annie Minerva Turnbo Malone died in Chicago, Illinois, at the age of 79, after suffering a stroke. Her death marked the end of an era for a woman who had made significant contributions to the beauty industry and African-American community. Her business and remaining fortune were bequeathed to her nieces and nephews.

Annie Turnbo married Aaron Eugene Malone, a former teacher and religious book salesman. This marriage had a significant impact on her business, Poro College, as her husband later demanded half of the business's value during their divorce in 1927.