The timber rattlesnake (Crotalus horridus), also known commonly as the banded rattlesnake, and the canebrake rattlesnake in the southern United States, is a species of pit viper in the family Viperidae. The species is native to the eastern United States. Like all other pit vipers, it is venomous, with a very toxic bite. Its venom is extremely potent, and both hemorrhagic and neurotoxic venom are present depending on population and location. C. horridus is the only rattlesnake species in most of the populous Northeastern United States and is one of the most northerly distributed venomous snakes in North America. There are no subspecies that are recognized as being valid.

The timber rattlesnake was one of the many reptile species originally described by Carl Linnaeus in the landmark 1758 10th edition of his Systema Naturae, and still bears its original name Crotalus horridus.

The subspecies C. h. atricaudatus (Latreille in Sonnini and Latreille, 1802), often referred to as the canebrake rattlesnake, is currently considered invalid. Previously, it was recognized by Gloyd (1936) and Klauber (1936). Based on an analysis of geographic variation, Pisani et al. (1972) concluded no subspecies should be recognized. This was rejected by Conant (1975), but followed by Collins and Knight (1980). Brown and Ernst (1986) found evidence for retaining the two subspecies, but stating them apart without having more information than usual is not possible, including adult size, color pattern, the number of dorsal scale rows, and the number of ventral scales. Dundee and Rossman (1989) recognized C. h. atricaudatus, but others take a more neutral point of view.

The timber rattlesnake is one of 36 species in the genus Crotalus. This genus can be distinguished from Sistrurus by the small scales atop the head, rather than nine large scales found on Sistrurus.

The name Crotalus is Latin for a rattle or, crŏtălisso to rattle or the sound of the castanet. Horridus is Latin, meaning horrid or dreadful, presumably in reference to its potentially lethal venom. Although southern populations are generally no longer recognized as a valid subspecies, the name canebrake rattlesnake (Crotalus horridus atricaudatus) is occasionally still used and commonly encountered in older literature. Atricaudatus is Latin meaning black-tailed (atri = black, and caudatus = tailed).

Adults usually grow to total length of 91–152 cm (36–60 in). In Pennsylvania, the smallest size females that could produce viable eggs was 72.2 cm (28.4 in). Most adult timber rattlesnakes found measure less than 115 cm (45 in) in total length and weigh between 500 and 1,500 g (1.1 and 3.3 lb), often being towards the lower end of that range. The maximum reported total length is 189.2 cm (74.5 in) (Klauber, 1956). Holt (1924) mentions a large specimen caught in Montgomery County, Alabama, which had a total length of 159 cm (62.5 in) and weighed 2.5 kg (5.5 lb). Large specimens can reportedly weigh as much as 4.5 kg (9.9 lb).

The dorsal scales are keeled and arranged in 21–26 scale rows at midbody (usually 25 rows in the southern part of its geographic range, and 23 rows in the northern part). The ventral scales number 158–177 in males and 163–183 in females. Males have 20–30 subcaudal scales, while females have 15–26. The rostral scale is normally a little taller than it is wide. In the internasal-prefrontal area there are 4–22 scales that include 2 large, triangular internasal scales that border the rostral, followed by two large, quadrangular prefrontal scales (anterior canthals) that may contact each other along the midline, or may be separated by many small scales. Between the supraocular and internasal scales, only a single canthal scale is present. Five to seven intersupraocular scales are seen. The number of prefoveal scales varies between two and eight. Usually, the first supralabial scale is in broad contact with the prenasal scale, although slightly to moderately separated along its posteroventral margin by the most anterior prefoveals.

Dorsally, they have a pattern of dark brown or black crossbands on a yellowish-brown or grayish background. The crossbands have irregular zig-zag edges, and may be V- or M-shaped. Often a rust-colored vertebral stripe is present. Ventrally, they are yellowish, uniform, or marked with black. Melanism is common, and some individuals are very dark, almost solid black. The tails of C. horridus are black and can be described as 'velvety.'