The Frauen DFB-Pokal 2000–01 was the 21st season of the cup competition, Germany's second-most important title in women's football. The first round of the competition was held on 19–20 August 2000. In the final which was held in Berlin on 26 May 2001 FFC Frankfurt defeated Flaesheim-Hillen 2–1, thus claiming their third title, all consecutive.[1] On their road to the final all games of Flaesheim-Hillen except the first had to be decided by a penalty shootout.
| 19 August 2000 | |||
| Fortuna Magdeburg-Wolmirstedt | 1 – 6 | Flaesheim-Hillen | |
| TSV Pfersee Augsburg | 0 – 5 | SC 07 Bad Neuenahr | |
| 1. FC Nürnberg | 1 – 5 | Bayern Munich | |
| SpVgg Rehweiler-Matzenbach | 0 – 4 | FSV Frankfurt | |
| 20 October 2000 | |||
| Berliner SV 92 | 0 – 16 | Turbine Potsdam | |
| FF USV Jena | 0 – 7 | Brauweiler Pulheim | |
| Hamburger SV | 2 – 1 | FCR 2001 Duisburg | (aet) |
| Turbine Potsdam II | 1 – 4 | Essen-Schönebeck | |
| DJK FSV Schwarzbach | 0 – 2 | 1. FC Saarbrücken | |
| PSV 90 Neubrandenburg | 1 – 13 | WSV Wolfsburg-Wendschott | |
| HSV Borussia Friedenstal | 5 – 0 | SV Neuenbrook/Rethwisch | |
| 1. FC Saarbrücken II | 0 – 7 | FFC Frankfurt | |
| TuS Niederkirchen | 0 – 2 | SC Freiburg | |
| SC 07 Bad Neuenahr II | 1 – 5 | FC Viktoria Neckarhausen | |
| SV Victoria Gersten | 2 – 5 | Heike Rheine | |
| ATS Buntentor | 0 – 5 | Sportfreunde Siegen |
| 5 November 2000 | |||
| Hamburg | 1 – 1 | Flaesheim-Hillen | (4–5 on penalties) |
| Essen-Schönebeck | 0 – 11 | Turbine Potsdam | |
| Heike Rheine | 1 – 2 | Brauweiler Pulheim | |
| Bayern Munich | 5 – 1 | 1. FC Saabrücken | |
| 1. FFC Frankfurt | 10 – 0 | Sportfreunde Siegen | |
| HSV Borussia Friedenstal | 1 – 5 | WSV Wolfsburg-Wendschott | |
| SC Freiburg | 2 – 2 | SC 07 Bad Neuenahr | (4–5 on penalties) |
| FC Viktoria Neckarhausen | 0 – 5 | FSV Frankfurt |
| 4 March 2001 | |||
| FSV Frankfurt | 3 – 2 | Bayern Munich | |
| WSV Wolfsburg-Wendschott | 2 – 3 | FFC Frankfurt | |
| SC 07 Bad Neuenahr | 2 – 2 | Turbine Potsdam | (5–6 on penalties) |
| 25 March 2001 | |||
| Flaesheim-Hillen | 0 – 0 | Brauweiler Pulheim | (3–2 on penalties) |
| 25 March 2001 | |||
| FSV Frankfurt | 1 – 2 | FFC Frankfurt | |
| Turbine Potsdam | 0 – 0 | Flaesheim-Hillen | (2–4 on penalties) |
| FFC Frankfurt | 2 – 1 | Flaesheim-Hillen |
|---|---|---|
Prinz  48'J. Meier 77' |
A. Meier 44' |
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
