Ayrton became actively involved in several suffrage organizations, including the Women's Social and Political Union (WSPU) and the National Union of Women's Suffrage Societies (NUWSS).

Ayrton provided financial support to the suffrage movement, contributing to the campaigns and activities of various organizations.

Ayrton sheltered suffragettes released from prison after hunger strikes, providing them with a place to recuperate and continue their activism.

Ayrton consistently advocated for women's rights, both within the scientific community and in society at large. She believed in equal opportunities for women in education and employment.