Holy Trinity Anglican Church is a heritage-listed Anglican church at 68 Hawthorne Street, Woolloongabba, Brisbane, Queensland, Australia. Since 1869, three church buildings have stood on this hill top site. The current church was completed in 1930. It was designed by the architect Eric Ford, featuring Romanesque and Spanish Mission Revival style architecture. Its preserved original architectural features make the church a traditional wedding venue of inner Brisbane. The church was added to the Queensland Heritage Register on 9 May 2008.

Woolloongabba is a densely occupied suburb of Brisbane, located 2 kilometres (1.2 mi) south of the CBD. Prior to the arrival of European people, Indigenous Australians lived in the area. These were people of the Turrbal nation.

In 1825, the Moreton Bay colony, a penal colony, was established on the north bank of the Brisbane River. Free settlement in the colony by European people was allowed from 1842. From that time until the 1860s, the area south of the river that would become Woolloongabba remained an undeveloped stretch of wetland known as One Mile Swamp.

In 1851, land in the One Mile Swamp area was made available for private purchase. In 1864, a set of residential and business allotments, called the Clarence Town Estate, was sold by auction. Advertisements for the allotments cited the benefits of an uninterrupted supply of fresh water from One Mile Swamp; the development of transportation and mail routes through the area to places like Cleveland and Ipswich; and the promise of a bridge across the Brisbane River.

In 1869, an allotment at Clarence Town Estate, atop a hill at Woolloongabba was donated to the Anglican Diocese of Brisbane by the Reverend Robert Gregory Creyke (born 9 June 1820, Yorkshire – died 11 February 1889, Cleveland, Queensland). Creyke held multiple roles in the colony: he was a minister of the Church of England; district registrar for Moreton Bay from 6 January 1860; and Queensland acting deputy registrar general. The deed of the allotment had been acquired in 1861 by his late son, also Robert Creyke, and Mary Ann Peterson. Meanwhile, a Church of England congregation had formed in the area. Its meetings and services were held in local buildings such as the Buffalo Hotel on the corner of Ipswich road and Hawthorne Street.

In 1870, a simple wooden church was built after a design by the Irish born, Australian architect, Richard Gailey (1834–1924). At this time, the church was called, Woolloongabba Church of England, South Brisbane. In 1873, the church was represented by Mr Robinson and children of the Sunday school at a meeting of the Church of England Sunday School Union in the City Botanic Gardens. This first church building blew down in a storm in October 1874.

A second church building was dedicated for service on 23 May 1875 by the Archdeacon Benjamin Glennie. It was known as Holy Trinity Church, Woolloongabba. It was built on a rectangular plan with a steeply pitched shingled roof and gables. Like the first church building, it was a rudimentary, timber structure. In their report of 1880, the Holy Trinity Churchwardens compared it to a woolshed. Improvements were difficult due to a lack of funds. The churchwardens went as far as raffling a chestnut horse in order to raise the monies needed. Eventually, the church ceiling was lined and a bell was installed. In 1884, a communion table was purchased.

The Reverend Hugh Thomas Molesworth (born June 1860, New Brompton, Kent – died 30 June 1930 Brisbane) was appointed curate-in-charge of Holy Trinity in 1886. At that time, Holy Trinity was a church within the Parish of South Brisbane but the congregation was growing. For instance, in 1886, the Holy Trinity Sunday School numbered 143 children and 24 teachers. Molesworth proposed the formation of a Woolloongabba parish, separate from that of South Brisbane. On 19 January 1888, the Holy Trinity Church of England Parish of Woolloongabba was constituted by the Brisbane Church of England Diocesan Council. Reverend David Ruddock (1850–1920) was appointed rector. He ministered at the church until 1893 and was replaced by the Reverend Hugh Simmonds.