Naismith worked as a physical education instructor at Springfield College. He taught various physical activities, including gymnastics, and developed innovative training methods.

Naismith held a professorship at the University of Kansas for nearly 40 years, where he taught physical education, hygiene, and other related subjects. He shaped the curriculum and mentored numerous students.

Naismith wrote articles and conducted research on physical education and sports. His writings reflected his belief in the importance of physical activity for overall health and well-being.

Following his retirement from the University of Kansas in 1937, Naismith was granted the title of professor emeritus, recognizing his long and distinguished service to the institution.