June A. McCarroll was born in Elkhorn, Walworth County, Wisconsin. Information regarding her parents and specific details of her early childhood are scarce, but this date marks the beginning of her life.

Records indicate June McCarroll received an education. The specific schools or institutions she attended during her formative years are not widely documented. However, she likely received a standard education for women of her time in rural Wisconsin.

Before pursuing a medical career, details of June McCarroll’s occupations are unknown. It is possible that she held various jobs common for women in that era, such as teaching or working in clerical positions, to support herself prior to her medical training.