Discrimination against lesbians, sometimes called lesbophobia or lesphobia, comprises various forms of prejudice and negativity towards lesbians as individuals, as couples, as a social group, or lesbianism in general.

Examples of discrimination against lesbians include, but are not limited to, discrimination in housing and employment, physical or sexual abuse including corrective rape, lack of legal protections for lesbian couples to care for one another, removal of children from lesbian mothers, negative stereotypes and negative media representation, verbal harassment, legal persecution and imprisonment, government censorship, and familial and/or community rejection.

Lesbophobia is analogous to gayphobia.

The first usage of the term lesbophobia listed in the Oxford English Dictionary is in The Erotic Life of the American Wife (1972), a book by Harper's Bazaar editor Natalie Gittelson. While some people use only the more general term homophobia to describe this sort of prejudice or behavior, others believe that the terms homosexual and homophobia do not adequately reflect the specific concerns of lesbians, because they experience the double discrimination of both homophobia and sexism.

Lesbophobia is sometimes demonstrated through crimes of violence, including physical assault, sexual assault, corrective rape and murder.

In the late 2000s, men murdered and raped several lesbians in South Africa. The victims included Sizakele Sigasa (a lesbian activist living in Soweto) and her partner Salome Masooa, who were raped, tortured, and murdered in an attack that South African lesbian-gay rights organizations, including the umbrella-group Joint Working Group, said were driven by lesbophobia. In the Gauteng township of KwaThema, soccer player Eudy Simelane was gang-raped, beaten and stabbed to death, and LGBTQ activist Noxolo Nogwaza was raped and stoned before being stabbed to death. Zanele Muholi, community relations director of a lesbian rights group, reports having recorded 50 rape cases over the past decade involving black lesbians in townships, stating: "The problem is largely that of patriarchy. The men who perpetrate such crimes see rape as curative and as an attempt to show women their place in society." Corrective rape is an ongoing social problem in South Africa.

In 1995, lesbian couple and gay rights activists Roxanne Ellis and Michelle Abdill were murdered in Oregon. Their killer stated that them being lesbians made it easier to kill them, and also had murdered a bisexual man for making a pass at him.

In its 2019 annual report, France's SOS Homophobie found that anti-lesbian violence increased 42 percent in France in 2018, with 365 attacks reported.