Lev Gleason Incorporated, formerly known as Lev Gleason Publishing, is a Canadian comic book company founded by Leverett Stone Gleason (1898–1971). They were the publisher of a number of popular comic books during the 1940s and early 1950s, including Daredevil Comics, Crime Does Not Pay, and Boy Comics. In 2021, Lev Gleason was revived by Fadi Hakim, and acquired the former Chapterhouse Comics and characters from Anglo-American Publishing.

Lev Gleason Publications was an influential comic book publisher active from the late 1930s to the mid-1950s. Founded by Leverett Stone Gleason, a pioneer in the comic book industry, the company was best known for its crime comics, particularly the best-selling series Crime Does Not Pay (1942–1955). Gleason began his publishing career after working with Charles Max Gaines at Eastern Color Printing, which played a significant role in the early days of comic books. He labelled some of his books ""illustories"" to suggest they were a new, unique form.

Gleason had a keen eye for talent, hiring notable creators such as Jack Cole and Charles Biro. Biro was instrumental in shaping the company’s creative direction. Gleason and Biro worked on various genres, including superhero, mystery, romance, comedy, western, and children's comics, reflecting their readership's diverse interests.

Gleason's career began in 1931 as an artist and advertising director for Open Road for Boys magazine. From 1932 to around 1934, he served as advertising manager under Harry Wildenberg at Eastern Color Printing, which became a comics-publishing pioneer in 1933 with the first American comic books. Gleason later worked as an editor at United Feature, where he launched Tip Top Comics in 1936. He then became business manager at Your Guide Publications, affiliated with Gilmor's Friday, Inc. and New Friday, Inc.

Around 1942, Gleason, then treasurer of New Friday, purchased the comic book series Silver Streak Comics and Daredevil Comics from the company. Under the imprint Comic House Inc., Gleason continued Silver Streak Comics with a crime comic, Crime Does Not Pay, which premiered with issue #22 in July 1942. That year, Gleason briefly published the left-wing political magazine Reader's Choice.

The success of Crime Does Not Pay led to numerous imitators and increased criticism of comic books' influence. This pressure resulted in the formation of the Association of Comics Magazine Publishers (ACMP) in 1948 to avoid external regulation, with Gleason as a founding member. The ACMP was the precursor to the Comics Code Authority, established in 1954.

In April 1949, Lev Gleason Publications, then located at 114 East 32nd Street in Manhattan, began publishing Tops, a comics magazine for adults. Despite its commercial success, Gleason's career was marked by controversy due to his progressive political views. As a member of the Communist Party in the 1930s, Gleason used his platform to promote social justice issues, making him a target during the post-WWII anti-communist campaigns, which contributed to the decline of his business.

Lev Gleason Publications went out of business in 1956. Its final publications were the teen-humor comic Jim Dandy #3 (Sept. 1956) and the children's Western comedy Shorty Shiner #3 (Oct. 1956), both published under the imprint Dandy Magazines Inc.