New Smyrna Beach is a city in Volusia County, Florida, United States, located on the central east coast of the state, with the Atlantic Ocean to the east. The downtown section of the city is located on the west side of the Indian River and the Indian River Lagoon system. The Coronado Beach Bridge crosses the Intracoastal Waterway just south of Ponce de Leon Inlet, connecting the mainland with the beach on the coastal barrier island. The population was 30,142 at the 2020 census; according to 2023 census estimates, the city is estimated to have a population of 32,655.

The surrounding area offers many opportunities for outdoor recreation; these include fishing, sailing, motorboating, golfing, and hiking. Visitors participate in water sports of all kinds, including swimming, scuba diving, kitesurfing, and surfing. In July 2009, New Smyrna Beach was ranked number nine on the list of "best surf towns" in Surfer. It was recognized as one of the "world's top 20 surf towns" by National Geographic in 2012. It has also been dubbed the "shark attack capital of the world."

New Smyrna Beach's motto is cygnus inter anates, which is Latin for "a swan among ducks."

The area was first settled by Europeans in 1768, when Scottish physician Dr. Andrew Turnbull, a friend of James Grant, the governor of British East Florida, established the colony of New Smyrna. Dr. Turnbull had married Gracia Dura Bin (some sources give her name as Maria Gracia Rubini), the daughter of a Greek London merchant from the Ottoman city of Smyrna (modern-day İzmir in Turkey) and named the settlement in honor of his wife's birthplace. (A number of the immigrants in his future labor force were Greeks from the Mani peninsula). No one had previously attempted to settle so many people at one time in a town in North America.

Turnbull recruited about 1,300 settlers, intending for them to grow hemp, sugarcane, and indigo, as well as to produce rum, at his plantation on the northeastern Atlantic coast of Florida. The majority of the colonists came from Menorca (historically called "Minorca" in English), one of the Mediterranean Balearic Islands of Spain, and were of Catalan culture and language.

Although the colony produced relatively large amounts of processed indigo in its first few years of operation, it eventually collapsed after suffering major losses due to insect-borne diseases and Indian raids, and growing tensions caused by mistreatment of the colonists on the part of Turnbull and his overseers. The survivors, about 600 in number, marched nearly 70 miles north on the King's Road and relocated to St. Augustine, where their descendants live to this day. In 1783, East and West Florida were returned to the Spanish, and Turnbull abandoned his colony to retire in Charleston, South Carolina.

The St. Photios Greek Orthodox National Shrine on St. George Street in St. Augustine honors the Greeks among the settlers of New Smyrna; they were the first Greek Orthodox followers in North America. The historical exhibit adjoining the chapel tells the story of their plight, with accompanying exhibits, and of their contributions to the city.

Central Florida remained sparsely populated by white settlers well into the 19th century, and it was frequently raided by Seminole Indians trying to protect their territory. United States troops fought against them in the Seminole Wars, but they were never completely dislodged.