Van Nistelrooy started his professional career at Den Bosch, scoring 17 goals in 69 appearances.

He then moved to Heerenveen, scoring 13 goals in 31 matches.

Van Nistelrooy joined PSV Eindhoven, where he won two Eredivisie titles and scored 62 goals in 67 appearances.

He moved to Manchester United, winning the Premier League, FA Cup, Football League Cup, and FA Community Shield, scoring 95 goals in 150 appearances.

Van Nistelrooy joined Real Madrid, winning La Liga twice and scoring 46 goals in 68 appearances.

He then moved to Hamburger SV, scoring 12 goals in 36 appearances.

Van Nistelrooy ended his playing career at Málaga, scoring 4 goals in 28 appearances.