Spider-Man: Into the Spider-Verse is a 2018 American animated superhero film based on the Marvel Comics character Spider-Man. Directed by Bob Persichetti, Peter Ramsey and Rodney Rothman from a screenplay by Phil Lord and Rothman, the film stars Shameik Moore as Miles Morales, with Jake Johnson, Hailee Steinfeld, Mahershala Ali, Brian Tyree Henry, Lily Tomlin, Luna Lauren Vélez, John Mulaney, Kimiko Glenn, Nicolas Cage and Liev Schreiber in supporting voice roles. The film's story follows Miles as he becomes the new Spider-Man and teams up with other Spider-People from various parallel universes to save his universe from the Kingpin. The first animated film in the Spider-Man franchise, it was produced by Sony Pictures Animation.

Plans for an animated Spider-Man film by Phil Lord and Christopher Miller were leaked in 2014 and announced in April 2015. Persichetti, Ramsey and Rothman joined over the next two years, with Moore and Schreiber cast in April 2017. Lord and Miller wanted the film to have a unique style, combining computer animation with hand-drawn comic techniques inspired by the work of Miles Morales co-creator Sara Pichelli. The film required the largest crew of animators used by Sony Pictures Animation on a feature film.

The film premiered at the Regency Village Theater in Los Angeles on December 1, 2018, and was theatrically released in the United States on December 14. The film grossed $394 million worldwide against a $90 million budget and received critical acclaim. The film won Best Animated Feature at the 91st Academy Awards, and numerous other accolades. Into the Spider-Verse has since been regarded as one of the greatest and most impactful animated films of all time, particularly for its groundbreaking achievement in animation, with filmmakers and animation peers praising its aesthetics and acknowledging its influence on subsequent animated productions.

A sequel, Spider-Man: Across the Spider-Verse, was released in June 2023 to similar critical acclaim and greater commercial success. A third film, Spider-Man: Beyond the Spider-Verse, is scheduled for release on June 18, 2027, rounding out the Spider-Verse trilogy, and spin-offs are in development.

New York City teenager Miles Morales struggles to live up to the expectations of his father, police officer Jefferson Davis, who sees Spider-Man as a menace. Miles' uncle Aaron takes him to an abandoned subway station to paint graffiti as a way to cheer him up. There, Miles is bitten by a radioactive spider and gains abilities similar to Spider-Man. Returning to the station where he got bit, he discovers a collider built by Kingpin, who hopes to access parallel universes to abduct alternate versions of his late wife Vanessa and son Richard. Spider-Man attempts to stop the collider while fighting Kingpin's enforcers, the Green Goblin and Prowler.

Green Goblin shoves Spider-Man into the collider, causing an explosion that mortally wounds Spider-Man, who then gives Miles a USB flash drive designed to sabotage the collider, warning that the machine could destroy the city if reactivated. After watching in horror as Kingpin murders Spider-Man, Miles flees. As the city mourns Spider-Man's death, Miles tries to honor his legacy by becoming the new Spider-Man, but inadvertently damages the drive. At Spider-Man's grave, he meets Peter B. Parker, a middle-aged and world-weary Spider-Man from another dimension.

The duo infiltrate Alchemax to steal data for a new flash drive. They are confronted by its proprietor, Olivia Octavius, who discovers that Peter will die from cellular decay if he remains in their dimension. Miles and Peter are saved by Gwen Stacy, a Spider-Woman from another dimension. They visit Spider-Man's aunt, May, who is sheltering more Spider-People who slipped into Miles' universe from other dimensions due to the collider's influence – Spider-Man Noir, Peni Parker and Spider-Ham – who are also deteriorating. Miles offers to help the others return home but they tell him he lacks experience.

Distraught, Miles retreats to Aaron's home, where he discovers his uncle is Prowler. He returns to the Parker house, where the new drive is completed; he is followed by Kingpin's team. In the ensuing brawl, he reveals his identity to Aaron. Unwilling to murder his own nephew, Aaron attempts to spare Miles, but is fatally shot by Kingpin in retaliation and dies in Miles' arms. Miles flees just as Jefferson arrives on the scene and mistakes the new Spider-Man as his brother's murderer before regrouping with the other Spider-People. Not wanting to let Miles get killed, Peter restrains Miles and chooses to sacrifice himself by staying behind and deactivating the collider.