The Jerusalem Talmud (Hebrew: תַּלְמוּד יְרוּשַׁלְמִי, romanized: Talmud Yerushalmi, often Yerushalmi for short) or Palestinian Talmud, also known as the Talmud of the Land of Israel, is a collection of rabbinic notes on the second-century Jewish oral tradition known as the Mishnah. Naming this version of the Talmud after Palestine or the Land of Israel—rather than Jerusalem—is considered more accurate, as the text originated mainly from Galilee in Byzantine Palaestina Secunda rather than from Jerusalem, where no Jews were allowed to live at the time.

The Jerusalem Talmud predates its counterpart, the Babylonian Talmud (known in Hebrew as the Talmud Bavli), by about a century. It was written primarily in Galilean Aramaic. It was compiled between the late fourth century to the first half of the fifth century. Both versions of the Talmud have two parts, the Mishnah (of which there is only one version), which was finalized by Judah ha-Nasi around the year 200 CE, and either the Babylonian or the Jerusalem Gemara. The Gemara is what differentiates the Jerusalem Talmud from its Babylonian counterpart. The Jerusalem Gemara contains the written discussions of generations of rabbis of the Talmudic academies in Syria Palaestina at Tiberias and Caesarea.

This version of the Talmud is frequently named the Jerusalem Talmud or the Palestinian Talmud. The latter name, after the region of Palestine – or the Land of Israel – is considered more accurate, as the text originated mainly from Galilee in Byzantine Palaestina Secunda rather than from Jerusalem, where no Jews lived at the time. The use of the parallel terms dates to the period of the geonim (6th–11th century CE), alongside other terms such as "Talmud of the Land of Israel", "Talmud of the West", and "Talmud of the Western Lands".

The Jerusalem Talmud probably originated in Tiberias in the School of Johanan bar Nappaha as a compilation of teachings of the schools of Tiberias, Caesarea, and Sepphoris.^{[citation needed]} It is written largely in Galilean Aramaic, a Western Aramaic dialect that differs from its Babylonian counterpart.

This Talmud is a synopsis of the analysis of the Mishnah that was developed for nearly 200 years by the Talmudic academies in Syria Palaestina (principally those of Tiberias and Caesarea). Because of their location, the sages of these Academies devoted considerable attention to the analysis of the agricultural laws of the Land of Israel.

The Leiden Jerusalem Talmud (Or. 4720) is today the only extant complete manuscript of the Jerusalem Talmud and available at Leiden University Libraries. It was copied in 1289 by Jehiel ben Jekuthiel Anav and shows elements of a later recension. The additions which are added in the biblical glosses of the Leiden manuscript do not appear in extant fragments of the same Talmudic tractates found in Yemen, additions which are now incorporated in every printed edition of the Jerusalem Talmud. These Yemenite fragments, a consequence of isolation the Yemenite community, are important as source material (as evidenced below).

The Leiden manuscript is important in that it preserves some earlier variants to textual readings, such as in Tractate Pesachim 10:3 (70a), which brings down the old Hebrew word for charoset (the sweet relish eaten at Passover), viz. dūkeh (Hebrew: דוכה), instead of rūbeh/rabah (Hebrew: רובה), saying with a play on words: "The members of Isse's household would say in the name of Isse: Why is it called dūkeh? It is because she pounds [the spiced ingredients] with him." The Hebrew word for "pound" is dakh (דך), which rules out the spelling of rabah (רבה), as found in the printed editions. Yemenite Jews still call it dūkeh. 

Leiden University Libraries has digitised both volumes of the manuscript and made it available in its Digital Collections.