The rotation curve of a disc galaxy (also called a velocity curve) is a plot of the orbital speeds of visible stars or gas in that galaxy versus their radial distance from that galaxy's centre. It is typically rendered graphically as a plot, and the data observed from each side of a spiral galaxy are generally asymmetric, so that data from each side are averaged to create the curve. A significant discrepancy exists between the experimental curves observed, and a curve derived by applying gravity theory to the matter observed in a galaxy. Theories involving dark matter are the main postulated solutions to account for the variance.

The rotational/orbital speeds of galaxies/stars do not follow the rules found in other orbital systems such as stars/planets and planets/moons that have most of their mass at the centre. Stars revolve around their galaxy's centre at equal or increasing speed over a large range of distances. In contrast, the orbital velocities of planets in planetary systems and moons orbiting planets decline with distance according to Kepler's third law. This reflects the mass distributions within those systems. The mass estimations for galaxies based on the light they emit are far too low to explain the velocity observations.

The galaxy rotation problem is the discrepancy between observed galaxy rotation curves and the theoretical prediction, assuming a centrally dominated mass associated with the observed luminous material. When mass profiles of galaxies are calculated from the distribution of stars in spirals and mass-to-light ratios in the stellar disks, they do not match with the masses derived from the observed rotation curves and the law of gravity. A solution to this conundrum is to hypothesize the existence of dark matter and to assume its distribution from the galaxy's center out to its halo. Thus the discrepancy between the two curves can be accounted for by adding a dark matter halo surrounding the galaxy.

Though dark matter is by far the most accepted explanation of the rotation problem, other proposals have been offered with varying degrees of success. Of the possible alternatives, one of the most notable is modified Newtonian dynamics (MOND), which involves modifying the laws of gravity.

Vesto Slipher made the first measurements related to galaxy rotation curves in 1914 when observing the Andromeda galaxy. Slipher observed that the stars on the left side of the galaxy's bulge were approaching at speeds of around 320 km/s, faster than those on the right, which were moving at about 280 km/s. This suggested that the galaxy's disc was rotating in such a way that it appeared to be spinning toward us.

In 1918 Francis Pease determined the rotation speed within the central region of Andromeda. His findings were represented by the formula ${\displaystyle \displaystyle V_{c}=-0.48r-316}$, where ${\displaystyle V_{c}}$ is the measured circular speed (in km/s) at a distance ${\displaystyle r}$ from the center of Andromeda's bulge. The results indicated that the central part of the galaxy rotates at a constant angular speed.

In 1932, Jan Hendrik Oort became the first to report that measurements of the stars in the solar neighborhood indicated that they moved faster than expected when a mass distribution based upon visible matter was assumed, but these measurements were later determined to be essentially erroneous. In 1939, Horace Babcock reported in his PhD thesis measurements of the rotation curve for Andromeda which suggested that the mass-to-luminosity ratio increases radially. He attributed that to either the absorption of light within the galaxy or to modified dynamics in the outer portions of the spiral and not to any form of missing matter. Babcock's measurements turned out to disagree substantially with those found later, and the first measurement of an extended rotation curve in good agreement with modern data was published in 1957 by Henk van de Hulst and collaborators, who studied M31 with the Dwingeloo Radio Observatory's newly commissioned 25-meter radio telescope. A companion paper by Maarten Schmidt showed that this rotation curve could be fit by a flattened mass distribution more extensive than the light. In 1959, Louise Volders used the same telescope to demonstrate that the spiral galaxy M33 also does not spin as expected according to Keplerian dynamics.

Reporting on NGC 3115, Jan Oort wrote that "the distribution of mass in the system appears to bear almost no relation to that of light... one finds the ratio of mass to light in the outer parts of NGC 3115 to be about 250". On page 302–303 of his journal article, he wrote that "The strongly condensed luminous system appears imbedded in a large and more or less homogeneous mass of great density" and although he went on to speculate that this mass may be either extremely faint dwarf stars or interstellar gas and dust, he had clearly detected the dark matter halo of this galaxy.