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Viking lander biological experiments
In 1976 two identical Viking program landers each carried four types of biological experiments to the surface of Mars. The first successful Mars landers, Viking 1 and Viking 2, then carried out experiments to look for biosignatures of microbial life on Mars. The landers each used a robotic arm to pick up and place soil samples into sealed test containers on the craft.
The two landers carried out the same tests at two places on Mars' surface, Viking 1 near the equator and Viking 2 further north.
The four experiments below are presented in the order in which they were carried out by the two Viking landers. The biology team leader for the Viking program was Harold P. Klein (NASA Ames).
A gas chromatograph – mass spectrometer (GCMS) is a device that separates vapor components chemically via a gas chromatograph and then feeds the result into a mass spectrometer, which measures the molecular weight of each chemical. As a result, it can separate, identify, and quantify a large number of different chemicals. The GCMS (PI: Klaus Biemann, MIT) was used to analyze the components of untreated Martian soil, and particularly those components that are released as the soil is heated to different temperatures. It could measure molecules present at a level of a few parts per billion.
The GCMS measured no significant amount of organic molecules in the Martian soil. In fact, Martian soils were found to contain less carbon than lifeless lunar soils returned by the Apollo program. This result was difficult to explain if Martian bacterial metabolism was responsible for the positive results seen by the Labeled Release experiment (see below). A 2011 astrobiology textbook notes that this was the decisive factor due to which "For most of the Viking scientists, the final conclusion was that the Viking missions failed to detect life in the Martian soil."
Experiments conducted in 2008 by the Phoenix lander discovered the presence of perchlorate in Martian soil. The 2011 astrobiology textbook discusses the importance of this finding with respect to the results obtained by Viking as "while perchlorate is too poor an oxidizer to reproduce the LR results (under the conditions of that experiment perchlorate does not oxidize organics), it does oxidize, and thus destroy, organics at the higher temperatures used in the Viking GCMS experiment. NASA astrobiologist Christopher McKay has estimated, in fact, that if Phoenix-like levels of perchlorates were present in the Viking samples, the organic content of the Martian soil could have been as high as 0.1% and still would have produced the (false) negative result that the GCMS returned. Thus, while conventional wisdom regarding the Viking biology experiments still points to "no evidence of life", recent years have seen at least a small shift toward "inconclusive evidence"."
According to a 2010 NASA press release: "The only organic chemicals identified when the Viking landers heated samples of Martian soil were chloromethane and dichloromethane—chlorine compounds interpreted at the time as likely contaminants from cleaning fluids." According to a paper authored by a team led by Rafael Navarro-González of the National Autonomous University of Mexico, "those chemicals are exactly what [their] new study found when a little perchlorate—the surprise finding from Phoenix—was added to desert soil from Chile containing organics and analyzed in the manner of the Viking tests." However, the 2010 NASA press release also noted that: "One reason the chlorinated organics found by Viking were interpreted as contaminants from Earth was that the ratio of two isotopes of chlorine in them matched the three-to-one ratio for those isotopes on Earth. The ratio for them on Mars has not been clearly determined yet. If it is found to be much different than Earth's, that would support the 1970s interpretation." Biemann has written a commentary critical of the Navarro-González and McKay paper, to which the latter have replied; the exchange was published in December 2011. In 2021 the chlorine isotope ratio on Mars was measured by the Trace Gas Orbiter and found to be almost indistinguishable from the terrestrial ratio, leaving the interpretation of the GCMS results inconclusive.
The gas exchange (GEX) experiment (PI: Vance Oyama, NASA Ames) looked for gases given off by an incubated soil sample by first replacing the Martian atmosphere with the inert gas helium. It applied a liquid complex of organic and inorganic nutrients and supplements to a soil sample, first with just nutrients added, then with water added too. Periodically, the instrument sampled the atmosphere of the incubation chamber and used a gas chromatograph to measure the concentrations of several gases, including oxygen, CO2, nitrogen, hydrogen, and methane. The scientists hypothesized that metabolizing organisms would either consume or release at least one of the gases being measured.
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Viking lander biological experiments
In 1976 two identical Viking program landers each carried four types of biological experiments to the surface of Mars. The first successful Mars landers, Viking 1 and Viking 2, then carried out experiments to look for biosignatures of microbial life on Mars. The landers each used a robotic arm to pick up and place soil samples into sealed test containers on the craft.
The two landers carried out the same tests at two places on Mars' surface, Viking 1 near the equator and Viking 2 further north.
The four experiments below are presented in the order in which they were carried out by the two Viking landers. The biology team leader for the Viking program was Harold P. Klein (NASA Ames).
A gas chromatograph – mass spectrometer (GCMS) is a device that separates vapor components chemically via a gas chromatograph and then feeds the result into a mass spectrometer, which measures the molecular weight of each chemical. As a result, it can separate, identify, and quantify a large number of different chemicals. The GCMS (PI: Klaus Biemann, MIT) was used to analyze the components of untreated Martian soil, and particularly those components that are released as the soil is heated to different temperatures. It could measure molecules present at a level of a few parts per billion.
The GCMS measured no significant amount of organic molecules in the Martian soil. In fact, Martian soils were found to contain less carbon than lifeless lunar soils returned by the Apollo program. This result was difficult to explain if Martian bacterial metabolism was responsible for the positive results seen by the Labeled Release experiment (see below). A 2011 astrobiology textbook notes that this was the decisive factor due to which "For most of the Viking scientists, the final conclusion was that the Viking missions failed to detect life in the Martian soil."
Experiments conducted in 2008 by the Phoenix lander discovered the presence of perchlorate in Martian soil. The 2011 astrobiology textbook discusses the importance of this finding with respect to the results obtained by Viking as "while perchlorate is too poor an oxidizer to reproduce the LR results (under the conditions of that experiment perchlorate does not oxidize organics), it does oxidize, and thus destroy, organics at the higher temperatures used in the Viking GCMS experiment. NASA astrobiologist Christopher McKay has estimated, in fact, that if Phoenix-like levels of perchlorates were present in the Viking samples, the organic content of the Martian soil could have been as high as 0.1% and still would have produced the (false) negative result that the GCMS returned. Thus, while conventional wisdom regarding the Viking biology experiments still points to "no evidence of life", recent years have seen at least a small shift toward "inconclusive evidence"."
According to a 2010 NASA press release: "The only organic chemicals identified when the Viking landers heated samples of Martian soil were chloromethane and dichloromethane—chlorine compounds interpreted at the time as likely contaminants from cleaning fluids." According to a paper authored by a team led by Rafael Navarro-González of the National Autonomous University of Mexico, "those chemicals are exactly what [their] new study found when a little perchlorate—the surprise finding from Phoenix—was added to desert soil from Chile containing organics and analyzed in the manner of the Viking tests." However, the 2010 NASA press release also noted that: "One reason the chlorinated organics found by Viking were interpreted as contaminants from Earth was that the ratio of two isotopes of chlorine in them matched the three-to-one ratio for those isotopes on Earth. The ratio for them on Mars has not been clearly determined yet. If it is found to be much different than Earth's, that would support the 1970s interpretation." Biemann has written a commentary critical of the Navarro-González and McKay paper, to which the latter have replied; the exchange was published in December 2011. In 2021 the chlorine isotope ratio on Mars was measured by the Trace Gas Orbiter and found to be almost indistinguishable from the terrestrial ratio, leaving the interpretation of the GCMS results inconclusive.
The gas exchange (GEX) experiment (PI: Vance Oyama, NASA Ames) looked for gases given off by an incubated soil sample by first replacing the Martian atmosphere with the inert gas helium. It applied a liquid complex of organic and inorganic nutrients and supplements to a soil sample, first with just nutrients added, then with water added too. Periodically, the instrument sampled the atmosphere of the incubation chamber and used a gas chromatograph to measure the concentrations of several gases, including oxygen, CO2, nitrogen, hydrogen, and methane. The scientists hypothesized that metabolizing organisms would either consume or release at least one of the gases being measured.