The Thirteenth Wisconsin Legislature convened from January 11, 1860, to April 2, 1860, in regular session.

Senators representing even-numbered districts were newly elected for this session and were serving the first year of a two-year term. Assembly members were elected to a one-year term. Assembly members and odd-numbered senators were elected in the general election of November 8, 1859. Senators representing odd-numbered districts were serving the second year of their two-year term, having been elected in the general election held on November 2, 1858.

The governor of Wisconsin during this entire term was Republican Alexander Randall, of Waukesha County, serving the first year of his second two-year term, having won re-election in the 1859 Wisconsin gubernatorial election.

Members of the Wisconsin Senate for the Thirteenth Wisconsin Legislature:

Members of the Assembly for the Thirteenth Wisconsin Legislature: