The 1965 Major League Baseball draft was the inaugural, amateur draft. It was held on June 8–9 in New York City.

The Kansas City Athletics selected Arizona State sophomore Rick Monday as the number one pick. Kansas City also chose ten future major leaguers, including Sal Bando (6th round) and Gene Tenace (20th round), building the base for the Oakland Athletics' championship teams of the early 1970s.

A total of 813 players were selected. Some of the more significant picks were catcher Johnny Bench by the Cincinnati Reds in the second round, pitcher Nolan Ryan by the New York Mets in the twelfth round, and infielder Graig Nettles by the Minnesota Twins in the fourth round. The first player to reach the majors was pitcher Joe Coleman, the Washington Senators' first pick and third pick overall. Future Hall of Famer Tom Seaver was selected by the Los Angeles Dodgers in the 10th round but did not sign and returned to the University of Southern California campus.

The list of later selections is limited to those who have made at least one major league appearance.