The 2001 NCAA Division I baseball tournament was played at the end of the 2001 NCAA Division I baseball season to determine the national champion of college baseball. The tournament concluded with eight teams competing in the College World Series, a double-elimination tournament in its fifty fifth year. Sixteen regional competitions were held to determine the participants in the final event, with each winner advancing to a best of three series against another regional champion for the right to play in the College World Series. Each region was composed of four teams, resulting in 64 teams participating in the tournament at the conclusion of their regular season, and in some cases, after a conference tournament. The fifty-fifth tournament's champion was Miami (FL), coached by Jim Morris. The Most Outstanding Player was Charlton Jimerson of Miami (FL).

Bold indicates CWS participant.

Bold indicates winner. * indicates extra innings. Italics indicates host.

The following players were members of the College World Series All-Tournament Team.