The 2016 Miami Open presented by Itaú (also known as 2016 Miami Masters) was a professional men and women's tennis tournament being played on outdoor hard courts. It was the 32nd edition of the Miami Open, and was part of the Masters 1000 category on the 2016 ATP World Tour, and of the Premier Mandatory category on the 2016 WTA Tour. All men and women's events took place at the Tennis Center at Crandon Park in Key Biscayne, Florida, United States, from March 21 through April 3, 2016. Novak Djokovic and Victoria Azarenka won the singles title.

The following are the seeded players. Rankings and seedings are based on ATP rankings as of March 21, 2016.

† The player used an exemption to skip the tournament in 2015. Accordingly, points for his 18th best result are deducted instead.
‡ The player did not qualify for the tournament in 2015. Accordingly, points for his 18th best result are deducted instead.

The following players received wildcards into the singles main draw:

The following players received entry using a protected ranking into the main draw:

The following players received entry from the qualifying draw:

The following players received entry as lucky losers:

The following pairs received wildcards into the doubles main draw: