| 2023 Santa Cruz Challenger | |
|---|---|
| Date | 11–17 September |
| Edition | 3rd |
| Surface | Clay |
| Location | Santa Cruz de la Sierra, Bolivia |
| Champions | |
| Singles | |
 Mariano Navone | |
| Doubles | |
 Boris Arias / Federico Zeballos | |
The 2023 Santa Cruz Challenger was a professional tennis tournament played on clay courts. It was the third edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 11 and 17 September 2023.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| ARG
|
Juan Manuel Cerúndolo | 93 | 1 |
| ARG
|
Genaro Alberto Olivieri | 140 | 2 |
| BOL
|
Hugo Dellien | 147 | 3 |
| ARG
|
Thiago Agustín Tirante | 150 | 4 |
| ARG
|
Francisco Comesaña | 160 | 5 |
| ARG
|
Mariano Navone | 184 | 6 |
 ITA
|
Luciano Darderi | 185 | 7 |
| ARG
|
Camilo Ugo Carabelli | 202 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw:
Mariano Navone def. Francisco Comesaña 4–6, 7–5, 6–1. Boris Arias / Federico Zeballos def. 
Matías Soto / Gonzalo Villanueva 6–2, 4–6, [10–7].
