The 2024 Lexington Challenger presented by Meridian Wealth Management was a professional tennis tournament played on outdoor hard courts. It was the twenty-ninth edition of the tournament for men, which was part of the 2024 ATP Challenger Tour, and the twenty-seventh edition for women, which was part of the 2024 ITF Women's World Tennis Tour. It took place in Lexington, Kentucky, United States, between 29 July and 4 August 2024, at the Hilary J. Boone Tennis Complex, a facility of the University of Kentucky.

The following players received a wildcard into the singles main draw:

The following player received entry into the singles main draw as a special exempt:

The following player received entry into the singles main draw as an alternate:

The following players received entry from the qualifying draw:

The following players received entry as lucky losers:

The following players received wildcards into the singles main draw:

The following player received entry into the singles main draw using a special ranking: