The 2024 Santa Cruz Challenger II was a professional tennis tournament played on clay courts. It was the fifth edition of the tournament which was part of the 2024 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 17 and 22 June 2024.

The following players received wildcards into the singles main draw:

The following player received entry into the singles main draw as a special exempt:

The following player received entry into the singles main draw using a protected ranking:

The following players received entry into the singles main draw as alternates:

The following players received entry from the qualifying draw:

The following player received entry as a lucky loser: