The activated sludge process is a type of biological wastewater treatment process for treating sewage or industrial wastewaters using aeration and a biological floc composed of bacteria and protozoa. It is one of several biological wastewater treatment alternatives in secondary treatment, which deals with the removal of biodegradable organic matter and suspended solids. It uses air (or oxygen) and microorganisms to biologically oxidize organic pollutants, producing a waste sludge (or floc) containing the oxidized material.

The activated sludge process for removing carbonaceous pollution begins with an aeration tank where air (or oxygen) is injected into the waste water. This is followed by a settling tank to allow the biological flocs (the sludge blanket) to settle, thus separating the biological sludge from the clear treated water. Part of the waste sludge is recycled to the aeration tank and the remaining waste sludge is removed for further treatment and ultimate disposal.

Plant types include package plants, oxidation ditch, deep shaft/vertical treatment, surface-aerated basins, and sequencing batch reactors (SBRs). Aeration methods include diffused aeration, surface aerators (cones) or, rarely, pure oxygen aeration.

Sludge bulking can occur which makes activated sludge difficult to settle and frequently has an adverse impact on final effluent quality. Treating sludge bulking and managing the plant to avoid a recurrence requires skilled management and may require full-time staffing of a works to allow immediate intervention. A new development of the activated sludge process is the Nereda process which produces a granular sludge that settles very well.

The activated sludge process is a biological process used to oxidise carbonaceous biological matter, oxidising nitrogenous matter (mainly ammonium and nitrogen) in biological matter, and removing nutrients (nitrogen and phosphorus).

The process takes advantage of aerobic micro-organisms that can digest organic matter in sewage, and clump together by flocculation entrapping fine particulate matter as they do so. It thereby produces a liquid that is relatively free from suspended solids and organic material, and flocculated particles that will readily settle out and can be removed.

The general arrangement of an activated sludge process for removing carbonaceous pollution includes the following items:

Treatment of nitrogenous or phosphorous matter comprises the addition of an anoxic compartment inside the aeration tank in order to perform the nitrification-denitrification process more efficiently. First, ammonia is oxidized to nitrite, which is then converted into nitrate in aerobic conditions (aeration compartment). Facultative bacteria then reduce the nitrate to nitrogen gas in anoxic conditions (anoxic compartment). Moreover, the organisms used for the phosphorus uptake (Polyphosphate Accumulating Organisms) are more efficient under anoxic conditions. These microorganisms accumulate large amounts of phosphates in their cells and are settled in the secondary clarifier. The settled sludge is either disposed of as waste activated sludge or reused in the aeration tank as return activated sludge. Some sludge must always be returned to the aeration tanks to maintain an adequate population of organisms.