Acts 13 is the thirteenth chapter of the Acts of the Apostles in the New Testament of the Christian Bible. It records the first missionary journey of Paul and Barnabas to Cyprus and Pisidia. The book containing this chapter is anonymous, but early Christian tradition uniformly affirmed that Luke composed this book as well as the Gospel of Luke. From this point onwards, except for the Council held in Jerusalem (Acts 15), Luke's narrative focusses on Paul, his ministry, and the events of his life.

The original text was written in Koine Greek. This chapter is divided into 52 verses.

Some early manuscripts containing the text of this chapter are:

The apostle Paul's "potted resume of Israel's history" in this chapter includes a number of Old Testament references:

This chapter mentions the following places (in order of appearance):

The first missionary journey of Paul and Barnabas took place about AD 47–48.

This section opens the account of Paul's first missionary journey (Acts 13:1-14:28) which starts with a deliberate and prayerful step of the church in Antioch, a young congregation established by those who had been scattered from persecution in Jerusalem (Acts 11:20–26) and has grown into an active missionary church. Paul's mission was not his own initiative, but was undertaken at the command of the Holy Spirit (verses 2, 4), with the framework of prayer and fasting forming an inclusio at the end of this first journey (Acts 14:26).

This Lucius of Cyrene is thought to be the same person as mentioned in Romans 16:21, or the same as Luke, the writer of the Gospel of Luke and the Acts of the Apostles. Heinrich Meyer observes that: