Acts 1 is the first chapter of the Acts of the Apostles in the New Testament of the Christian Bible. Early Christian tradition affirmed that Luke composed this book as well as the Gospel of Luke. Critical opinion on the tradition was evenly divided at the end of the 20th century. This chapter functions as a transition from the "former account" (that is, the Gospel of Luke) with a narrative prelude (verses 1–5), repeated record of the ascension of Jesus Christ with more detail (verses 6–11) and the meeting of Jesus' followers (verses 12–26), waiting until Pentecost.

The original text was written in Koine Greek. This chapter is divided into 26 verses.

Some early manuscripts containing the text of this chapter are:

This chapter mentions the following places:

The beginning of the book offers a conventional opening statement containing the name of the addressee, Theophilus, and a brief reminder of the content of the "former account" (the Gospel of Luke) by the same author.

Forty days after his resurrection, Jesus commands the disciples during a meal to stay in Jerusalem and to wait for the coming of the Holy Spirit. The Greek: συναλιζομενος (sunalizomenos) is rendered as "he was eating with them" in the New International Version. Some translations state that they were "assembled" or "gathered" together. Whether "eating" represents the correct interpretation has been long debated.

Then a cloud takes Jesus upward from sight, and two men in white appear to tell them (the disciples) that he will return "in the same way you have seen him go into heaven".

Before Jesus left, he charged the disciples with the task of acting as 'witnesses' to him, in the locations that can be read as a 'geographical program' for the whole book of Acts: