Following her graduation, Aslan started her career as a researcher. She worked in various hospital settings and focused on research on the effects of procaine.

Aslan became a professor at the Institute of Geriatrics in Bucharest. This position allowed her to further develop her research and teaching in the field of gerontology.

Aslan developed Gerovital H3, a drug that she believed could alleviate the effects of aging. This marked a turning point in her career and established her as a leading figure in gerontology. The drug contains procaine, which Aslan believed had rejuvenating properties. Her research indicated that it had positive effects on symptoms related to aging.

For the next several decades, Aslan dedicated herself to the research and promotion of Gerovital H3. She conducted extensive clinical trials, presented her findings at international conferences, and published numerous articles on its potential benefits.

Ana Aslan founded the Institute of Gerontology and Geriatrics in Bucharest, Romania. This institute became a leading center for research on aging and age-related diseases.