Ana Aslan (born Anna Aslanyan) was born in Brăila, Romania, to a family of partial Armenian descent. Her early life was marked by a strong familial influence, which instilled in her a sense of purpose and dedication.

Aslan showed an early aptitude for learning. Details about her primary and secondary education are somewhat sparse, but it's clear she excelled academically, demonstrating a keen interest in scientific subjects.

Ana Aslan pursued medical studies at the University of Bucharest, a crucial period in shaping her future career. Her dedication during World War I led to her assisting in front-line hospitals, gaining invaluable practical experience and solidifying her commitment to medicine.