Against Me! is an American punk rock band, formed in 1997 in Naples, Florida, by singer and guitarist Laura Jane Grace. That same year, Grace moved to Gainesville, Florida, which is considered the band's hometown. Since 2001, the band's lineup has also included guitarist James Bowman, who has played on all of their studio albums. After releasing three studio albums through independent record labels, Against Me! moved to Sire Records for 2007's New Wave, which reached no. 57 on the Billboard 200. In 2011, the band launched the record label Total Treble.

In 2012, Grace publicly came out as a trans woman. After a long period of line-up changes and uncertainty surrounding the band's future, their sixth studio album, Transgender Dysphoria Blues, was released independently in January 2014 to critical and commercial acclaim. The band's seventh full-length, Shape Shift with Me, was released September 16, 2016.

Following the release of Shape Shift with Me, longtime bassist Andrew Seward rejoined the group in 2018. Though the group intended to record a new album, plans were derailed by the COVID-19 pandemic and the band entered an indefinite hiatus.

Against Me! was formed in 1997 by singer/guitarist Laura Jane Grace as a solo act, amidst the breakup of her previous band(s). The first Against Me! release was an eponymously titled demo tape in 1997. It was self-released in very limited numbers. With the addition of drummer Kevin Mahon, a second demo, Vivida Vis! (1998), was released in limited numbers by Misanthrope Records. The band started their first tour in March 1999, a month-long tour of the East coast, during which they met Jordan Kleeman in Baltimore, Maryland. The band's first EP, Against Me!, was released twice through Crasshole Records (once in 1999 and 2000). Grace's longtime friend James Bowman joined the band for a west coast tour in support of the EP, traveling in Grace's 1976 Buick LeSabre, the engine of which exploded at the end of the tour. Their first widely distributed release was the EP Crime as Forgiven by Against Me! (2001), released by Plan-It-X Records and Sabot Productions (run by longtime Against Me! tour manager Jordan Kleeman). While neither Bowman or Fridkin had part in the recording of Crime as Forgiven by Against Me!, they both joined the band on tour to support it. On April 23, 2001, while heading south on I-75, returning home from their first tour as a four-piece band, their van was struck by a semi truck. They landed upside-down in a ditch. No one was harmed, but the van and equipment were destroyed, after which Bowman and Mahon left the band. The band broke up for a few months that year. A third EP, a self-titled acoustic recording by Grace and bassist Dustin Fridkin, was released in November 2001.

Mahon was eventually replaced by Warren Oakes, and guitarist James Bowman returned in December 2001 during the recording of the band's first album, Against Me! Is Reinventing Axl Rose, released in March 2002 by No Idea Records. Fridkin left during the summer of 2002 to return to college and was replaced by Andrew Seward of the Tennessee band kill devil hills, and this new lineup released the single "The Disco Before the Breakdown" (2002). This lineup remained intact until 2009.

Against Me! signed to Fat Wreck Chords and released their second album, 2003's Against Me! as the Eternal Cowboy. It reached no. 36 on Billboard's Top Independent Albums chart and was supported by singles for "Cavalier Eternal" and "Sink, Florida, Sink". The DVD We're Never Going Home followed, documenting their Spring 2004 tour. 2005's Searching for a Former Clarity was their first album to chart on the Billboard 200, reaching no. 114. Against Me! supported the album with a tour of all 50 U.S. states. It spawned singles for "Don't Lose Touch" and "From Her Lips to God's Ears (The Energizer)", which also became the band's first music videos. The live album Americans Abroad!!! Against Me!!! Live in London!!! followed in 2006.

Against Me! moved to Sire Records for 2007's New Wave, produced by Butch Vig. It debuted at no. 57 on the Billboard 200 and featured their first charting single, "Thrash Unreal", which reached no. 11 on Billboard's Modern Rock Tracks chart. "White People for Peace", "Stop!", and "New Wave" were also released as singles, and New Wave was named as Spin's album of the year. The DVD Live at the Key Club followed later that year. In 2008, Against Me! supported Foo Fighters on a North American tour, and Sire released the digital EP New Wave B-Sides. In October 2008, while traveling west on I-80, en route to Salt Lake City, their tour bus hit a patch of ice; causing it to spin and land on its side in a ditch. No one was harmed.

For the 2008 presidential election, Against Me! partnered with Rock the Vote.