Anna Wagner Keichline was born in Bellefonte, Pennsylvania, to John A. and Emma Keichline. Her upbringing in a rural environment, coupled with her father's hardware store and lumber yard, likely sparked her early interest in construction and design, setting the stage for her future career.

After graduating from Bellefonte High School in 1906, Keichline worked for a year as a clerk for her father. Rather than going directly to architecture school, she apprenticed with architects, which was the typical form of architecture education during that period.

Anna Keichline distinguished herself by earning a Bachelor of Science degree in Mechanical Engineering from Pennsylvania State College (now Penn State University). This was an unusual feat for a woman at the time and provided her with a strong technical foundation for her architectural pursuits.

Following her graduation from Penn State, Keichline broadened her knowledge of architecture and design by working with multiple architects, including at least one in New York City. This was a significant step in acquiring practical skills and broadening her artistic and technical understanding of building design.

Keichline achieved a major milestone by becoming a registered architect in Pennsylvania. This accomplishment allowed her to independently design and oversee building projects, solidifying her professional status and opening doors to greater opportunities.

During the 1920s, Keichline devoted time to her inventions, most of which were intended to improve domestic spaces. She focused on improving airflow and space efficiency with things such as a combination sink and washbasin for small spaces, a folding bed, and improved ventilation, reflecting her interest in practical and innovative solutions.

Keichline obtained a patent for her innovative 'K-Brick,' a hollow fireproof brick with interlocking grooves designed for efficient building and concealed wiring. This invention garnered considerable attention and highlighted her inventive spirit and understanding of building materials.

During World War I, Anna Keichline served as a Special Agent for the U.S. government. Her engineering background and attention to detail made her valuable in investigating industrial sabotage and security threats. This role showcased her patriotism and commitment to national service.

Anna Wagner Keichline passed away on February 5, 1943, in her hometown of Bellefonte, Pennsylvania. Despite her relatively short life, she left behind a legacy of innovation, architectural contributions, and a testament to the power of women in male-dominated fields. Her impact continues to inspire those seeking to break barriers and make a difference.