Born to John Irvin Keichline and Emma Wagner Keichline in Bellefonte, Pennsylvania. She grew up in a supportive family environment that encouraged her intellectual pursuits.

Maintained close ties with her family and the local community in Bellefonte. Her family's involvement in construction and business likely influenced her career path.

It is believed that Anna Keichline remained unmarried throughout her life. The limited historical record has not provided any evidence of marital status. However, some speculation is possible based on some historical information.

Historical references occasionally suggest that Anna Keichline was a lesbian, a topic often obscured due to historical biases. This conclusion may be based on some gender non-conformities. However, there is no definitive historical record to verify.

Towards the end of her life, Anna Keichline faced health problems. Anna Keichline died in 1943. Given her relatively young age at the time of her death, further investigations are needed to find out her death cause.