An ant supercolony is an exceptionally large ant colony, consisting of a high number of spatially separated but socially connected nests of a single ant species (meaning that the colony is polydomous), spread over a large area without territorial borders. Supercolonies are typically polygynous, containing many egg-laying females (queens or gynes). Workers and queens from different nests within the same supercolony can freely move among the nests, and all workers cooperate indiscriminately with each other in collecting food and care of the brood, and show no apparent mutual aggressive behavior.

As long as suitable unoccupied space with sufficient resources is available, supercolonies expand continuously through budding, as queens together with some workers migrate over short distances and establish a new connected nest. The supercolony can also expand over long distances through jump-dispersal, potentially ranging between continents. Jump-dispersal usually occurs unintentionally through human-mediated transport. A striking example of an ant species forming supercolonies across continents is the Argentine ant (Linepithema humile). The also highly invasive red imported fire ant (Solenopsis invicta) and Solenopsis geminata additionally use classic mating flights, thus using three primary modes of dispersal. Out of some 14,000 described ant species, supercolonialism is found in less than 1% of all ants.

In general, ants that form supercolonies are invasive and harmful in the non-native environments. While not all supercolonial species are invasive and not all invasive ants are dominant, supercolonies are usually associated with invasive populations. Some invasive species are known to form supercolonies in their native habitat as well. In their native range, relatively small supercolonies are observed, whereas they are much larger, dominant and a threat for ecological diversity in their invasive range. Exceptions of species that form supercolonies without being invasive are mainly found in the genus Formica.

Although supercolonies are mainly observed in relatively few ant species, similar unicolonial populations are also found in some species of the termite genus Reticulitermes.

Initially, it was hypothesized that unicoloniality is a characteristic of certain ant species in which all workers of that species are amicable, whatever their nest of origin. So, all members of the species would accept each other, irrespective of the nest of origin and irrespective of the distance between the nests. In contrast, multicoloniality is the common characteristic of ants to show all colonies being aggressive to each other, including different colonies of the same species. A supercolony would be a large aggregation of nests of a species that normally would exhibit multicoloniality, but in the case of a supercolony has all workers from all connected nests being non-aggressive to each other. The Argentine ant (Linepithema humile), forming megacolonies of spatially separate nests, was thought to be a perfect example of unicoloniality, never exhibiting multicoloniality.

Giraud et al. (2002), however, discovered that L. humile also forms supercolonies that are aggressive to each other, so unicoloniality turned out to be limited. They hypothesized that the difference between supercoloniality and unicoloniality is not clear-cut, but that they are rather points on a continuum between two extremes: multicoloniality with all colonies generally being aggressive to each other, contrasted with unicoloniality with absolute absence of aggression between colonies, and supercoloniality somewhere in between.

Therefore, Pedersen et al. (2006) redefined supercoloniality and unicoloniality as follows:

Supercolony: A colony that contains such a large number of nests that direct cooperative interactions are impossible between individuals in distant nests. There are no behavioral boundaries (aggression) within the supercolony.