Ascot Vale is an inner-city suburb in Melbourne, Victoria, Australia, 6 km (3.7 mi) north of Melbourne's Central Business District, located within the City of Moonee Valley local government area. Ascot Vale recorded a population of 15,197 at the 2021 census.

Ascot Vale is bounded in the west by the Maribyrnong River, in the north by Maribyrnong and Ormond Roads, in the east by the Moonee Ponds Creek, and in the south by Lyons Road, Epsom Road to the railway line thence generally north-east to Moonee Ponds Creek.

Ascot Vale West Post Office opened on 1 January 1888 and was renamed Ascot Vale around 1893. An Ascot Vale East office was open from 1914 until 1979.

The Temperance Township Estate in Ascot Vale, bounded by Union, Maribyrnong and Epsom Roads, was established with a caveat on each title preventing the distilling, brewing, or the serving of alcoholic beverages, but hotels were soon built at the outside corners of the settlement.

The following places in Ascot Vale are listed on the Victorian Heritage Register:

In the 2021 census, there were 15,197 people in Ascot Vale. 67.2% of people were born in Australia. The next most common countries of birth were Vietnam 2.6%, England 2.3%, Italy 2.0%, China 1.6% and New Zealand 1.5%. 69.2% of people only spoke English at home. Other languages spoken at home included Italian 3.1%, Vietnamese 3.1%, Greek 2.1%, Cantonese 1.9% and Arabic 1.6%. The most common responses for religion were No Religion 41.5% and Catholic 27.0%.

A major landmark in the suburb is the Melbourne Showgrounds, which has special events such as the annual Royal Melbourne Show. The Showgrounds are adjacent to the Flemington Racecourse which is in neighbouring Flemington.

Ascot Vale has the Ascot Vale Special School, Ascot Vale Primary School, Ascot Vale West Primary School, St Mary's Catholic Primary School Ascot Vale and the arts program of Western Autistic School.