Australians in Japan comprise Australian citizens residing in Japan. In December 2023, there were 13,015 resident Australians in Japan. This figure does not include individuals naturalised as Japanese citizens, short-term residents, or Japanese people with Australian ancestry who do not hold Australian citizenship. Japan does not allow its citizens over 18 to have dual citizenship.

Tokyo, Hokkaido, Nagano, Osaka and Kanagawa are major population centres for Australians in Japan.

Since the early 2000s, a significant Australian community has emerged in the Niseko area of Hokkaido, which comprises the towns of Kutchan, Niseko, and Rankoshi. This is due to the popularity of the region's ski resorts. In 2020, 33.1% of foreign nationals living in Kutchan were Australians. Many Australian settlers have established tourism-related businesses such as guest houses and restaurants, and holiday homes in the area, and the area has been described as a 'Little Australia'.