The Biltmore Conference, also known by its resolution as the Biltmore Program, was a fundamental departure from traditional Zionist policy by its demand "that Palestine be established as a Jewish Commonwealth." The meeting was held in New York City, at the prestigious Biltmore Hotel, from May 6 to May 11, 1942, with 600 delegates and Zionist leaders from 18 countries attending.

The program has been described by a number of historians as "a virtual coup d’état" within Zionism since the movement's more moderate leaders were replaced by leaders with more aggressive goals.

Prior to this Extraordinary Zionist Conference at the Biltmore, official Zionism had steadfastly refused to formulate the ultimate aim of the movement but preferred to concentrate on the practical task of building the Jewish National Home. The Biltmore Program became the official Zionist stand on the ultimate aim of the movement. According to Ben-Gurion, the "first and essential" stage of the program was the immigration of two million additional Jews to Palestine. In 1944, a One Million Plan would become the official policy of the Zionist leadership. There was no reference to the Arab population prevailing in Mandatory Palestine, which, according to Anita Shapira, marked a transition to the view that conflict was inevitable between the Arabs and Jews and could only be resolved by the sword.

The major shift at Biltmore was prompted by intense common opposition to the British White Paper of 1939, which interpreted the terms of the Mandate in a way that would freeze "the Jewish community to a permanent minority status", and the negative situation during the war. The shift was prompted also by the realization that America would play a larger part in fulfillment of Zionist designs after the war.

Official Zionism’s firm unequivocal stand did not please everyone, however. The pro-British Chaim Weizmann had bristled at it. Also, binationalists such as Henrietta Szold and Judah L. Magnes rejected the stand and broke off to establish their own party, Ichud ("Unification"), which advocated an Arab–Jewish federation. Opposition to the Biltmore Program also led to the founding of the anti-Zionist American Council for Judaism.

Various Zionist organizations were represented in the American Emergency Committee of Zionist Affairs, which called an "Extraordinary Zionist Conference" as a substitute for the full (22nd) Zionist Congress, which had been cancelled because of World War II. Attendees included Chaim Weizmann, as President of the Zionist Organization; David Ben-Gurion, as Chairman of the Jewish Agency Executive; and Nahum Goldmann, as a member of the Executive of the Zionist Organization of America. The four main organisations of American Jewry represented were the Zionist Organization of America, Hadassah, Mizrahi, and Poale Zion.^{[citation needed]} Among the American organizers was Reform Rabbi Abba Hillel Silver.^{[citation needed]}

The joint statement issued at the end of the session was known as the Biltmore Program. It asked for unrestricted Jewish immigration to Palestine. Here is the full text of the program:

After approval by the Zionist General Council in Palestine, the Biltmore Program was adopted as the platform of the World Zionist Organization.