Burlington Trailways was an intercity bus company based in West Burlington, Iowa.

After 96 years of operations, Burlington announced it would cease scheduled intercity bus operations in September 2025, while shop services concluded all projects during December.

Burlington Trailways was founded in 1929 as the Burlington Transportation Company, a subsidiary of the Chicago, Burlington and Quincy Railroad. It started as a bus line that ran through Highway 34. In 1934, the service expanded to Denver and Omaha and in 1935, from Chicago to California. In 1936, it was a charter member of the Trailways Transportation System, an association of independent intercity bus operators created to offset the growing strength of Greyhound Lines.

In 1952, Burlington Transportation Company was renamed into Burlington Trailways.

As of September 2022, Burlington Trailways operates the following routes. Intermediate stops are not listed.