Calenzano (Italian pronunciation: [kalenˈdzaːno]) is a comune (municipality) in the Metropolitan City of Florence in the Italian region Tuscany, located about 11 kilometres (7 mi) northwest of Florence. As of 31 December 2004, it had a population of 15,557 and an area of 76.9 square kilometres (29.7 mi²).

Calenzano borders the following municipalities: Barberino di Mugello, Campi Bisenzio, Prato, San Piero a Sieve, Sesto Fiorentino, Vaglia, Vaiano.

Calenzano has a station on the regional railway connecting it to Prato, Florence, Pistoia and Lucca. It can be reached by road from the A1 Motorway. Bus services connect it to Prato, Florence, Campi Bisenzio and Barberino di Mugello.