The Chautauqua Institution (/ʃəˈtɔːkwə/ shə-TAW-kwə) is a 501(c)(3) nonprofit education center and summer resort for adults and youth located on 2,070 acres (840 ha) in Chautauqua, New York, 17 miles (27 km) northwest of Jamestown in the western southern tier of New York state. Established in 1874, the institution was the home of, and provided the impetus for, the Chautauqua movement that became popular in the United States in the late 19th and early 20th centuries. The Chautauqua Institution Historic District is listed on the National Register of Historic Places and was further designated a National Historic Landmark.

Chautauqua was founded in 1874 by inventor Lewis Miller and Methodist Bishop John Heyl Vincent as a teaching camp for Sunday-school teachers. The teachers would arrive by steamboat on Chautauqua Lake, disembark at Palestine Park and begin a course of Bible study that used the Park to teach the geography of the Holy Land.

The institution has operated each summer since then, gradually expanding its season length and program offerings in the arts, education, religion, and music. It offers educational activities to the public during the season, with public events including popular entertainment, theater, symphony, ballet, opera, and visual arts exhibitions. The institution also offers a variety of recreational activities, plus a community education program called Special Studies along with residential programs of intensive study provided for students aiming for professional careers who audition for admittance into Chautauqua's Schools of Performing and Visual Arts.

The physical setting of the institution defined its development as an assembly. The grounds are on the west shoreline of upper Chautauqua Lake. The early tent-camp assembly gave way to cottages and rooming houses, and then hotels, inns and eventually condominiums.

Founder Lewis Miller's daughter, Mina Miller Edison (wife of inventor Thomas Edison) offered literary classes in Fort Myers, Florida, through the Valinda Society. After completing courses, students were given Chautauqua diplomas.

In 1973, the National Park Service added the Institution to the National Register of Historic Places. In 1989, the Department of the Interior designated it a National Historic Landmark District consisting of most of the Institution property between NY 394, formerly NY 17J, the lake and (roughly) Lowell and North Avenues.

Every summer during its nine-week season, Chautauqua Institution provides an array of programs including fine and performing arts, lectures, worship services, and religious programs, as well as recreational activities. Nearly 100,000 visitors come to Chautauqua to participate in these programs and events annually. Summer admission to Chautauqua is by "gate ticket," which allows entrance into the grounds, use of Smith Memorial Library, use of public beaches and parks, and attendance at lectures and concerts. There is an additional charge for some courses, for films shown at the Chautauqua Cinema, for opera and theater tickets, and use of the tennis courts and golf courses. Cottages and rooms are available for long or short term rental.

Programs offered during the week at Chautauqua include devotional services and a lecture on a social, political, or academic issue in the morning, a religious or political topic in the afternoon, and a night of entertainment as the evening program. This evening Amphitheater event may be a symphony concert by the Chautauqua Symphony Orchestra, a dance program by the Chautauqua Ballet Company, or a show by an individual guest artist. During most weeks, there is at least one opportunity to catch an opera and a play put on by Chautauqua's resident summer companies. Operas are performed in English at Norton Hall, a 1930s-era, art-deco structure. There are also regularly scheduled organ recitals on the Massey Memorial Organ, student recitals, masterclasses, forums, and seminars for the sophisticated.