The following are fictional characters from Disney's 1940 film Fantasia, its 1999 sequel Fantasia 2000, and the 2014 video game Fantasia: Music Evolved. Characters are sorted by the film and segment in which they appear.

Mickey Mouse appears as the main character of the segment The Sorcerer's Apprentice in both Fantasia and Fantasia 2000.

Yen Sid, whose name is derived from "Disney" spelled backwards, is a sorcerer who appears in The Sorcerer's Apprentice. In the segment, his apprentice, Mickey Mouse, watches him cast spells before he decides to take a break, leaving his hat behind. Mickey takes the hat and uses its magic to bring a broom to life to help him retrieve water from the well. However, he is unable to control the magic, and the basement floods after he falls asleep. Eventually, Sid arrives and undoes the spell. Afterwards, he takes back his hat and broom and sends Mickey to finish his chores.

Donald Duck appears as a main character in the segment Noah's Ark (Pomp and Circumstance – Marches 1, 2, 3 and 4) from Fantasia 2000.

Daisy Duck appears as a main character in the segment Noah's Ark (Pomp and Circumstance – Marches 1, 2, 3 and 4) from Fantasia 2000.

Chernabog is a devil who appears in Night on Bald Mountain, who can take people's hearts and holds power over various restless souls. His name is derived from Chernobog, a deity from Slavic mythology. While officially a pagan god, Chernabog may have been intended as a representation of Satan, with Walt Disney and Deems Taylor referring to him as such.

In the segment, on Walpurgis Night, Chernabog is disguised as the spire atop Bald Mountain until he reveals himself and uses his powers to raise various evil spirits and restless souls. However, they retreat after morning dawns, signified by the tolling of the Angelus Bell.

This section excludes Toccata and Fugue in D Minor, as no characters appear in them.