Doublecortin-like kinase protein 1 (DCLK1) is an enzyme that in humans is encoded by the DCLK1 gene. Its C-terminal domain in rats is expressed independently from an alternative transcript, cpg16, and can function alone as a serine/threonine protein kinase that is cyclic AMP dependent.

DCLK1 expression is a marker for tuft cells, a type of chemosensory cell of the intestinal epithelium.