In many Methodist Churches, a District Conference is a unit of church organisation with respect to hierarchy.

In the Evangelical Wesleyan Church, district conferences are required to be held thrice a year. It is presided over by a bishop. It grants and renews licenses to preach, recommends suitable local preachers to the annual conference, recommends suitable deaconesses to the annual conference, among other responsibilities.

In the Primitive Methodist Church, a district superintendent presides over the district conference.

In the United Methodist Church, district conferences consist of clergy from the district, as well as lay delegates from each pastoral charge. It is presided over by a District Superintendent.