The Florida Central and Peninsular Railroad was the final name of a system of railroads throughout Florida, becoming part of the Seaboard Air Line Railway in 1900. The system, including some of the first railroads in Florida, stretched from Jacksonville west through Tallahassee and south to Tampa. Much of the FC&P network is still in service under the ownership of CSX Transportation.

The Tallahassee Rail Road was first organized in 1832 as the Leon Railway, changing its name in 1834. It opened in 1837, connecting Tallahassee, Florida to the Gulf of Mexico port of St. Marks, Florida. This was the second steam railroad in Florida, opening just a year after the Lake Wimico and St. Joseph Canal and Railroad.

The Florida, Atlantic and Gulf Central Railroad was chartered January 24, 1851, to build west from Jacksonville, Florida, and construction began in 1857. The Pensacola and Georgia Railroad was chartered in January 1853, to be built east from Pensacola, Florida, but started at Tallahassee. The two lines met at Lake City, Florida in 1860, and the latter also built from Tallahassee west to four miles (6 km) short of Quincy, Florida, stopping in 1863 in the middle of the American Civil War.

In 1855 the Pensacola and Georgia bought the Tallahassee. In 1869 the two merged to form the Jacksonville, Pensacola and Mobile Railroad, which obtained trackage rights over the Florida Central Railroad, the 1868 reorganization of the Florida, Atlantic and Gulf. The railroad eventually was built west to Chattahoochee, Florida, a major junction with the Pensacola and Atlantic Railroad continuing west and the Chattahoochee and East Pass Railroad running northeast. In 1882, Sir Edward Reed purchased the Jacksonville, Pensacola and Mobile, absorbing the Florida Central and reorganizing the two as the Florida Central and Western Railroad.

The Florida Railroad was incorporated January 8, 1853, to build a line across the state, from Fernandina, Florida (north of Jacksonville, Florida) southwest to Cedar Key, Florida. The first train ran in 1861, but the line failed and the company was reorganized in 1866. In 1872 it was reorganized again as the Atlantic, Gulf and West India Transit Company. In 1881, Sir Edward Reed purchased the railroad and reorganized it as the Florida Transit Company, which in 1883 was reorganized again as the Florida Transit and Peninsular Railroad. The Florida Transit and Peninsular operated two subsidiaries, the Peninsula Railroad and Tropical Florida Railroad, organized to build lines respectively from the Florida Transit at Waldo, Florida to Ocala, Florida and beyond to Tampa, Florida.

In 1884-85, Reed merged the Florida Transit and Peninsular Railroad with the Florida Central and Western Railroad, Fernandina and Jacksonville Railroad, and Leesburg and Indian River Railroad as the Florida Railway and Navigation Company, which instantly became the largest railroad system in Florida. The new company was placed in receivership in October 1885, sold at foreclosure and reorganized as the Florida Central and Peninsular Railroad Company in 1886.

On May 1, 1889, the company was reorganized again, as the Florida Central and Peninsular Railway, and on January 16, 1893, the final reorganization produced the Florida Central and Peninsular Railroad, along with a merger of the Florida Northern Railroad (a line from Yulee to Savannah, Georgia). The Seaboard Air Line Railway leased the FC&P on July 1, 1900, and the latter was merged into the former on August 15, 1903. The FC&P tracks from Savannah, Georgia to Tampa, Florida via Jacksonville became part of Seaboard's main line.

Though a series of mergers between 1967 and 1987, the Seaboard became part of CSX Transportation. Much of the former FC&P network remains in service today.