Florida State Road 121
Florida State Road 121
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Florida State Road 121

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Florida State Road 121

State Road 121 (SR 121) is a major state highway that runs north and south in northern Florida. The road is part of a long multi-state route that also goes through Georgia and South Carolina.

SR 121 is one of the major routes of a three-state highway 121 that totals approximately 475 miles (764 km) from Lebanon Station, Florida, to Rock Hill, South Carolina.

Beginning in Lebanon Station at a channelized intersection with US 19/US 98 and County Road 336 (CR 336) in Levy County, SR 121 travels through Goethe State Forest where it turns east and intersects CR 337. The road makes a sharp left curve to the northeast where it travels through desolate farmland; the only resemblance to a major intersection is CR 326. Within the city of Williston, SR 121 has a concurrency with CR 316, until the intersection with US 41. It then joins US 41 as CR 316 continues eastward, which then joins the east end of US 27 Alternate (US 27 Alt.), and then the US 27/US 41 concurrency before breaking away to the northeast again, taking the DeSoto Trail away from US 41.

North of CR 335, SR 121 hugs the west side of the Levy–Marion county line, but never crosses it, choosing to enter Alachua County instead. Immediately after the interchange with Interstate 75 (I-75) at exit 382, SR 121 makes a sharp left onto Northwest 34th Street as SR 331 becomes the designation for Williston Road. As it crosses SR 24 it borders the western edge of the University of Florida, passing along the 34th Street Wall just before crossing SR 26A and then SR 26. North of US 441, the DeSoto Trail breaks away.

Within the town of LaCrosse, SR 121 shares a short concurrency with SR 235, then has a level crossing with the CSX Brooker Subdivision, a former Seaboard Coast Line railroad line that leads to the Crystal River 3 Nuclear Power Plant. After the intersection with CR 236 in Santa Fe, SR 121 passes by entrances to the Santa Fe River Preserve, and then is carried along a bridge over the Santa Fe River in Worthington Springs, where it intersects SR 18. North of CR 18, the road is carried along a bridge over the former Jacksonville and Southwestern Railroad.

Briefly traveling parallel to SR 100 in Lake Butler, it intersects SR 100 as it continues northeast. The road also runs parallel to another rail-trail along the former Jacksonville and Southwestern right-of-way in Raiford, then serves as the western terminus of SR 16 before leaving that community. After passing by the Northeast Florida State Hospital, the road intersects I-10 (Exit 335) and US 90 in Macclenny close to SR 228 before crossing the Georgia state line on its way to South Carolina.

The segment between US 19 and 98 in Lebanon Station and US 41 in Williston was previously designated as Florida State Road 335, and the segment between US 27 and 41 in Williston, at what is today the south end of SR 331 was part of a southern extension of that route.
State Road 23 was defined in the 1945 renumbering as:

Prior to the renumbering, this had been:

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