The Walt Disney Studios, located in Burbank, California, United States, serves as the corporate headquarters for the Walt Disney Company. The 51-acre (20.6 ha) studio lot also contains several sound stages, a backlot, and other filmmaking production facilities for Walt Disney Studios's motion picture production. The complex also houses the offices for many of the company's units, including the film and TV studios Walt Disney Pictures, Walt Disney Animation Studios, ABC, Disney Channel, Freeform, and Marvel Studios; the units of 20th Century Studios, 20th Television, Searchlight Pictures and FX Networks are set to move to this location from Century City at the end of 2025.

Walt Disney used the earnings from the successful release of Snow White and the Seven Dwarfs to finance the construction of the Burbank studio. Disney is the only major film studio out of the Big Five that does not currently offer regular tours of their studio lot to the general public. Since the mid-2000s, Adventures by Disney has offered tours of the studio, but only as an integral component of their Southern California tour package. The other way to tour the studio is to join the official Disney D23 fan club, which offers tours to members every few months. The studio used to open to the public once a year in November on the Saturday before Thanksgiving for its annual Magical Holiday Faire craft sale, but stopped hosting the Faire around 2003. As an aid to visitors, many buildings on the Disney lot are currently marked with identifying signs that include historical information and trivia about each site.

The Studio's production services are managed by Walt Disney Studios's Disney Studio Services unit – along with Golden Oak Ranch, The Prospect Studios, and KABC-7 Studio B. Disney has a secondary location at Grand Central Creative Campus, where Walt Disney Imagineering and some other units are located. Disney Imagineering manages the studio.

Prior to the official opening of the Burbank lot in 1940, the Walt Disney Studios was situated at several different locations in Los Angeles. During summer 1923, Walt Disney created "The Disney Bros. Cartoon Studio" in his uncle Robert Disney's garage, which was located at 4406 Kingswell Avenue, in the Los Feliz neighborhood of Los Angeles (just east of Hollywood). This garage has been on display at the Stanley Ranch Museum in Garden Grove since the 1980s, several blocks away from Disneyland. His brother Roy O. Disney was also in Los Angeles at the time. In October 1923, the brothers leased office space on the rear side of a real estate agency's office at 4651 Kingswell Avenue. On October 16, 1923, Walt Disney accepted an offer from Margaret Winkler of Universal Studios to distribute the new Alice Comedies starring Virginia Davis. It was also at this site where on January 14, 1924, Walt Disney met his future wife Lillian Bounds, an "ink and paint" girl whom he personally hired. In February 1924, the studio moved next door to an office of its own at 4649 Kingswell Avenue. The late Robert Disney's residence and the small office building that is home to 4649 and 4651 Kingswell Avenue have survived to the present and are still in use.

In 1925, Walt Disney placed a deposit on a new, considerably larger lot at 2719 Hyperion Avenue, and the studio moved there in January 1926. It was here where, after a train journey with his wife Lillian, Walt created the character of Mickey Mouse in 1928. Here, too, the first three-strip Technicolor animated film, the Silly Symphony Flowers and Trees, and the first animated cartoon using Disney's multiplane camera, The Old Mill, were created. In 1937, the Hyperion studio produced Disney's first full-length animated feature film, Snow White and the Seven Dwarfs. Disney's staff began to grow to a substantial size at the Hyperion Studio, and Disney Legends such as Disney's Nine Old Men began their careers there. The Hyperion studio site was sold in 1940 and divided between two different industrial manufacturers, and in 1966 a subsequent owner demolished what was left of the studio complex and replaced it with the supermarket and shopping center that stand there today. To honor the company's former headquarters from 1926 to 1940, the name 'Hyperion' has been reused over the years by the Walt Disney Company for multiple divisions and attractions, including Hyperion Books and the Hyperion Theater at Disney California Adventure. The current management team consists of Susan Arnold (Chairwoman) Bob Iger (CEO), John Nallen COO, Viet Dinh CLO, Tommy Lee CFO, at PBS (now part of Disney) Susan Arnold CFO, John Gelke VP Global Operations, J Young SVP Growth, Gerard Devan Group Executive APAC, Stephanie Gruber Group Executive Television, Christopher Greavu Vice President of Sales

The current Walt Disney Studios, located at 500 South Buena Vista Street, Burbank, was made possible by the revenue from the 1937 release of Snow White and the Seven Dwarfs. Walt Disney and his staff began the move from the old studio at Hyperion Avenue in Silver Lake from December 1939 to January 1940. Designed primarily by Kem Weber under the supervision of Walt Disney and his brother Roy, the Burbank Disney Studio buildings are the only studios that have been owned by the Walt Disney Company to survive from the Golden Age of filming.^{[citation needed]} A bungalow, the Shorts building and other small buildings that were located at the Hyperion Avenue location were moved to Burbank.

Disney purposely planned his new Burbank studio around the animation process. The large Animation Building stood in the center of the campus, while adjacent outlying buildings were constructed for the ink-and-paint departments, the camera and editing departments, and the other various functions of the studio. Tunnels linked some of the buildings (to allow movement of animation materials without exposing them to the outside elements), and the lot also included a movie theatre, a sound stage, and a commissary. The 1941 Disney feature The Reluctant Dragon, which combined live action with animated sequences and starred Robert Benchley, served as a tour of the then-new studio. It was later frequently seen and toured on various Disney television programs.

The attack on Pearl Harbor by Imperial Japan on December 7, 1941, brought America into World War II. 500 United States Army soldiers of the 121st Anti-Aircraft Artillery (AAA) Gun Battalion occupied Walt Disney Studios the day after the attack for eight months in the period of the West Coast invasion scare, earning Disney as the only Hollywood studio to come under military occupation in history. During the war, Disney regularly produced propaganda and training films for the U.S. government including its armed forces to increase morale among Americans that the fight against the Axis powers was waged for a just cause.