The Galleria Borghese or Borghese Gallery is an art gallery in Rome, Italy, housed in the former Villa Borghese Pinciana. At the outset, the gallery building was integrated with its gardens, but nowadays the Villa Borghese gardens are considered a separate tourist attraction. The Galleria Borghese houses a substantial part of the Borghese Collection of paintings, sculpture and antiquities, begun by Cardinal Scipione Borghese, the nephew of Pope Paul V (reign 1605–1621). The building was constructed by the architect Flaminio Ponzio, developing sketches by Scipione Borghese himself, who used it as a villa suburbana, a country villa at the edge of Rome.

The gallery displays one of the most prestigious art collections in the world, with masterpieces by artists such as Caravaggio, Bernini, Canova, Raphael and Titian. Scipione Borghese was an early patron of Bernini and an avid collector of works by Caravaggio, who is well represented in the collection by his Boy with a Basket of Fruit, St Jerome Writing, Sick Bacchus and others. Additional paintings of note include Titian's Sacred and Profane Love, Raphael's Entombment of Christ and works by Peter Paul Rubens and Federico Barocci. Considered among the greatest masterpieces of Italian art, some of these works show the evolution of art between the Renaissance, the Baroque and the Neoclassicism, artistic movements born in the Italian peninsula and subsequently spread throughout Europe.

The Casino Borghese was erected in an area that in the seventeenth-century was outside the walls of Rome, with the closest access being the Porta del Popolo. At the origins, the villa grounds covered an area with a circumference of nearly 5 km (3 miles).

The main building was designed by the Flemish architect Giovanni Vasanzio. The portico had spolia derived from the Arch of Claudius, once on the Via Flaminia.

By 1644, John Evelyn described it as "an Elysium of delight" with "Fountains of sundry inventions, Groves and small Rivulets of Water". Evelyn also described the Vivarium that housed ostriches, peacocks, swans and cranes "and divers strange Beasts". Prince Marcantonio IV Borghese (1730–1800), who began the recasting of the park's formal garden architecture into an English landscape garden, also set out about 1775, under the guidance of the architect Antonio Asprucci, to replace the now-outdated tapestry and leather hangings and renovate the Casina, restaging the Borghese sculptures and antiquities in a thematic new ordering that celebrated the Borghese position in Rome. The rehabilitation of the much-visited villa as a genuinely public museum in the late eighteenth century was the subject of an exhibition at the Getty Research Institute, Los Angeles, in 2000, spurred by the Getty's acquisition of 54 drawings related to the project.

In 1808, Prince Camillo Borghese, Napoleon's brother-in-law, was forced to sell the Borghese Roman sculptures and antiquities to the Emperor. The result is that the Borghese Gladiator, renowned since the 1620s as the most admired single sculpture in Villa Borghese, must now be appreciated in the Louvre. The "Borghese Hermaphroditus" is also now in the Louvre.

The Borghese villa was modified and extended down the years, eventually being sold to the Italian government in 1902, along with the entire Borghese estate and surrounding gardens and parkland.

The Galleria Borghese includes twenty rooms across two floors.