Girls of the Big House is a 1945 American drama film directed by George Archainbaud and starring Lynne Roberts, Virginia Christine and Marion Martin.

The film's sets were designed by the art director Gano Chittenden.

A college professor's daughter is convicted of a crime she didn't commit. Under an assumed name, Jeanne Crail, she is imprisoned with inmates including Bernice Meyers, who misses her boyfriend Smiley, and the condemned Alma Vlasek, who killed a policeman while waiting to ambush her cheating husband and his mistress.

Jeanne breaks out of jail to go see her sweetheart, lawyer Bart Sturgis. When her man Smiley visits prison, Bernice is infuriated by his attraction to Jeanne and attacks her with a knife. Jeanne ends up in solitary confinement. Alma, finally realizing who her husband's secret lover was, murders Bernice in the prison. Bart's efforts help clear Jeanne's name and win her release.